Embryonic stem cell test(EST)was widely used in evaluation of embryotoxicity or developmental toxicity of compounds in the fields of drugs,chemicals,and environmental safety.In recent years.Compared to the traditional reproductive toxicity tests,EST has the advantages of short cycletime,high throughput and low loss,in order to further improve the applicability and safety assessment of EST to various drugs and to avoid species differences,researchers used human embryonic stem cell(hESC)instead of the original mouse embryonic stem cell(mESC),and the researches which utilized hESC to evaluate embryotoxicity or developmental toxicity have been became more diversified and in-depth,such as the development of different evaluation models of differentiation types(myocardial cell,nerve cell and osteoblast etc.)or the construction of three-dimensional(3D)models which were closer to real living condition of embryo(embryoid bodys and organoid),these studies provided a new direction for establishing a more accurate and efficient model for the evaluation of embryotoxicity.
关键词
人胚胎干细胞/胚胎,哺乳动物/模型,理论/细胞,培养的/体外培养技术/胚胎毒性
Key words
Human embryonic stem cells/Embryo,mammalian/Models,theoretical/Cells,cultured/In vitro techniques/Embryotoxicity
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