Effect of Lupeol on Proliferation,Migration and Invasion of Lung Adenocarcinoma Cells by Targeting CDC25A
Objective To analyze the effect of lupeol on proliferation,migration,invasion of lung adenocarcinoma cells by targeting CDC25A.Methods The targets of lupeol and their correla-tion with clinical phenotypes of lung adenocarcinoma were analyzed by bioinformatics.A549 and Calu-3 cells were divided into 6 groups:control group,lupeol group,vector group,lupinol+vec-tor group,silence group and lupinol+silence group.The cells activity,migration and invasion were detected.The xenograft models of nude mice with lung adenocarcinoma were constructed to observe the effects of lupeol on volume and weight of xenograft tumors,and expression levels of Ki67 and CDC25A proteins.The relationship between expression level of CDC25A protein and the concentra-tion and time of lupeol treatment was measured.Results Bioinformatic analysis showed that CDC25A might be one of lupeol targets,and the expression level of CDC25A was closely related to the progression of lung adenocarcinoma.The survival rates and colony numbers of A549 and Calu-3 cells were decreased with the increase of lupeol concentration(P＜0.05).The number of migration and invasion lung adenocarcinoma cells in the lupeol group were significantly lower than those in the control group(P＜0.05),the volume and weight of xenograft tumors,the expression levels of Ki67 and CDC25A were significantly lower than those in the control group(P＜0.05).The expres-sion level of CDC25A was significantly decreased with the increase of time and concentration of lupe-ol treatment(P＜0.05).The expression level of CDC25A,the cells activities,the number of colo-nies and migration and invasion cells in the lupinol+vector group,the silence group and the lupinol+silence group were significantly lower than those in the vector group(P＜0.05).Conclusion Lu-peol can inhibit malignancy of lung adenocarcinoma tumor cells by down-regulating CDC25A.
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