Mechanism of Schisantherin B Inhibiting Proliferation of Uterine Cor-pus Endometrial Carcinoma Cells and Epithelial-mesenchymal Transi-tion by Targeting PTGS1
Objective To analyze the mechanism of Schisantherin B inhibiting the proliferation of uterine corpus endometrial carcinoma(UCEC)cells and epithelial-mesenchymal transition(EMT)by targeting prostaglandin-endoperoxide synthase 1(PTGS1).Methods The target(PTGS1)of Schisantherin B was predicted by TargetNet.The relationship between the expression of PTGS1 and the clinicopathological characteristics of UCEC was analyzed by TCGA database.The control group,Schisantherin B group and sh-PTGS1 group were set up.The survival and colony for-mation abilities of cells,and the expression levels of EMT associated proteins were detected.The effect of PTGS1 on volume and weight of xenograft tumors,and the level of proliferation index(Ki67)were observed.Ishikawa and HEC108 cell lines with stable overexpression of PTGS1 were constructed.The effect of Schisantherin B on survival,colony formation abilities,and expression levels of EMT proteins of Ishikawa and HEC108 cells with PTGS1 overexpression were detec-ted.Results Compared with those in the control group,the proliferation and numbers of cell colo-nies,and the expression levels of PTGS1,Vimentin,N-cadherin and Snail in the sh-PTGS1 group and the Schisantherin B group were significantly decreased,the expression level of E-cadherin in the above two groups was significantly increased;the volume and weight of xenograft tumor tissues,and the expression level of Ki67 in the sh-PTGS1 group were significantly decreased(P＜0.05).However,the expression level of E-cadherin was significantly decreased in the PTGS1 overexpres-sion group,the expression levels of PTGS1,Vimentin,N-cadherin,Snail,and the cell viabili-ties,and number of cell colonies of Ishikawa and HEC108 cells were significantly increased when compared with those in the control group(P＜0.05).In addition,when compared with those in the Schisantherin B group,the expression level of E-cadherin was significantly decreased in the Schisandrin B+PTGS1 overexpression group,while the expression levels of PTGS1,Vimentin,N-cadherin,Snail were significantly increased(P＜0.05).Conclusion Schisantherin B may in-hibit the proliferation and EMT of UCEC cells by down-regulating PTGS1.
Schisantherin Buterine corpus endometrial carcinomacell proliferationepi-thelial mesenchymal transitionprostaglandin-endoperoxide synthase 1
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