Anti-tumor Effect of Tetramethylpyrazine-containing Serum on Liver Cancer HepG2 Cells
Objective To explore the effect and mechanism of tetramethylpyrazine(TMP)-containing serum on proliferation,apoptosis and mitochondrial function of liver cancer cells.Methods Serum from SD rats treated with 0,30,60,120 mg/kg of TMP was collected and used to treat cells.Colony formation experiment and CCK-8 were employed to test the proliferation of HepG2 cells.Flow cytometry was performed to detect HepG2 cell cycle and apoptosis,mitochondrial membrane potential changes of HepG2 cells were measured by JC-1 through flow cytometry.Western blotting was used to detect the expression levels of PCNA,P27,cleaved caspase-3(cas-3),Bax,Bcl-2 and Cyt C in HepG2 cells.SOD and MDA levels in HepG2 cells were detected by kits,and 2,7-dichlorodiacetate(DCFH-DA)was used to determine the production of reactive oxygen spe-cies(ROS)in HepG2 cells.Results Compared with that of the control group,the TMP-contai-ning serum of 60 and 120 mg/kg TMP groups significantly reduced the proliferation of HepG2 cells,and blocked cell cycle,moreover,HepG2 cell apoptosis rate was significantly increased and the antioxidant capacity was significantly reduced.Conclusion TMP-containing serum can inhibit the proliferation of liver cancer cells and induce apoptosis of liver cancer cells through a mitochondrial-dependent pathway,and stimulate the generation of ROS to reduce the antioxidant capacity of canc-er cells,thereby inhibiting the development of liver cancer.
liver cancertetramethylpyrazineproliferationcell apoptosismitochondriareactive oxygen species
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