目的 探究肌球蛋白重链11(myosin heavy chain 11,MYH11)对喉鳞状细胞癌细胞恶性生物学行为的影响及机制.方法qRT-PCR检测MYH11 mRNA在喉鳞状细胞癌组织以及喉鳞状细胞癌细胞中的表达.喉鳞状细胞癌细胞TU686分为si-MYH11组和si-NC组.FD-LSC-1细胞分为MYH11组和Vector组.CCK8、流式细胞术、细胞划痕、Transwell实验分别用于检测细胞增殖、凋亡、迁移以及侵袭能力,蛋白质印迹检测各组细胞ERK 1/2磷酸化水平及MAPK相对表达量.结果 MYH11高表达于喉鳞状细胞癌组织及细胞.si-MYH11组TU686细胞增殖、迁移和侵袭能力显著低于si-NC组(P均＜0.05),细胞凋亡显著高于si-NC组(P＜0.01),ERK1/2磷酸化水平及MAPK表达显著低于si-NC组(P＜0.01).MYH11组TU686细胞增殖、迁移和侵袭能力显著高于Vector组(P均＜0.05),细胞凋亡显著低于Vector组(P＜0.01),ERK1/2磷酸化水平及MAPK表达显著高于Vector组(P＜0.01).结论 MYH11激活ERK/MAPK信号通路而促进喉鳞状细胞癌细胞的增殖、迁移和侵袭能力.
MYH11 Activates the ERK/MAPK Signaling Pathway and Induces Growth and Metastasis of Laryngeal Squamous Cell Carcinoma Cells
Objective To investigate the effect and mechanism of myosin heavy chain 11(MYH11)on the malignant biological behaviors of laryngeal squamous cell carcinoma cells.Methods qRT-PCR was used to detect the expression level of MYH11 mRNA in laryngeal squamous cell carcinoma tissues and cells.Laryngeal squamous cell carcinoma cells TU686 were di-vided into 2 groups:si-MYH11 group and si-NC group.FD-LSC-1 cells were divided into 2 groups:MYH11 group and Vector group.CCK8,flow cytometry,wound-healing assay and Transwell assay were used to determine the cell proliferation,apoptosis,migration,and invasion abilities,respec-tively.Western blotting was used to detect the phosphorylation level of ERK1/2 and the expression level of MAPK in each group of cells.Results MYH11 was highly expressed in laryngeal squamous cell carcinoma tissues and cells.The proliferation,migration,and invasion ability of TU686 cells in the si-MYH11 group were significantly lower than those in the si-NC group(P＜0.05),and cell apoptosis was significantly higher than that in the si-NC group(P＜0.01),the ERK1/2 phospho-rylation level and MAPK expression level were significantly lower than those in the si-NC group(P＜0.01).The proliferation,migration,and invasion ability of TU686 cells in the MYH11 group were significantly higher than those in the Vector group(P＜0.05),and cell apoptosis was signifi-cantly lower than that in the Vector group(P＜0.01),the phosphorylation level of ERK1/2 and MAPK expression level were significantly higher than those in the Vector group(P＜0.01).Con-clusion MYH11 activates the ERK/MAPK signaling pathway and promotes the proliferation,mi-gration,and invasion of laryngeal squamous cell carcinoma cells.
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