Screening of methylation driving genes in renal clear cell carcinoma and its prognostic role
Objective To screen methylation driving genes of renal clear cell carcinoma,con-struct prognostic molecular label based on methylation driving genes of renal clear cell carcinoma,and explore its prognostic role in renal clear cells.Methods The gene expression data,DNA methylation data and clinical information of renal clear cell carcinoma in TCGA database were downloaded.The MethylMix package of the R language was used to comprehensively analyze the patient's gene expres-sion data and DNA methylation data to screen methylation driving genes,and then the transcriptome data of 70％patients with renal clear cell carcinoma were randomly selected.The prognostic molecular tags were constructed by LASSO-Cox regression,and the constructed molecular tags were verified by 30％data.Results According to the expression profile data and methylation data of renal clear cell carcinoma,188 methylation driving genes were screened(Cor＜-0.3,all P＜0.05),104 were hypomethylation driving genes and 84 hypermethylation driving genes;Based on ccrcc transcriptome data and survival data,91 of the 188 methylation driving genes were found to be related to the progno-sis of ccrcc(P＜0.05).Combined with the clinical prognosis data,eight methylation driver genes EPB41L4B,GOLGA6L2,HHLA2,HOXA2,LINC00944,SPINT2,ZNF382 and ZNF888 related to the prognosis of renal clear cell carcinoma were screened by lasso Cox regression analysis.The prognostic molecular tag was constructed by combining eight methylation driver genes:Risk score=0.130004056 × EXPEPB41L4B+0.110026099 × EXPGOLGA6L2-0.116610796 × EXPHHLA2+0.438033838 × EX-PHOXA2+0.180898961 × EXPLINC00944-0.168803405 × EXPSPINT2-0.326061874 × EX-PZNF3821-0.152001589 × EXPZNF888.Survival curve analysis results showed that the constructed molecular tag value was significantly correlated with the survival of ccRCC patients in the training group,the test group and all data groups(all P＜0.05),and the higher the molecular tag value,the worse the prognosis of patients.Conclusions By mining of TCGA database,188 methylation driving genes of renal clear cell carcinoma were screened,of which 91 methylation driving genes were related to the prognosis of ccRCC,and a prognostic molecular tag composed of 8 genes is screened from 91 genes related to the prognosis of renal clear cell carcinoma,the constructed prognostic molecular tag has a significant correlation with the prognosis of patients with renal cell carcinoma,which provides a new prognostic model for the precise treatment of renal cell carcinoma.
DNA MethylationMethylation-Driven GenesClear Cell Carcinoma of Kidney
NETL
NSTL
万方数据
维普
