The expressions of peripheral blood lncRNA ANRIL and association with left ventricular hyper-trophy in patients with diabetic kidney disease
Objective To examine the expressions of long noncoding RNA ANRIL(lncRNA ANRIL)in peripheral blood with diabetic kidney disease(DKD)patients,and to explore the correla-tion between ANRIL and left ventricular hypertrophy(LVH).Methods From January 2020 to De-cember 2021,66 DKD patients(DKD group)and 20 normal healthy people(control group)were col-lected.Expressions of lncRNA ANRIL in peripheral blood of all participants were measured by RT-qPCR.DKD patients were divided into non-LVH group(23 cases)and LVH group(43 group)accord-ing to left ventricular mass index(LVMI).The differences of ANRIL expressions were examined in the two groups.The logistic regression was explored to analyze the risk factor of LVH in DKD patients.Receiver operating characteristic(ROC)curve was used to evaluate the value of lncRNA ANRIL in predicting LVH in DKD patients.Results The systolic blood pressure(SBP),diastolic blood pressure(DBP),serum creatinine(Scr),blood urea nitrogen(BUN)and cystatin C(Cys C)in DKD patients were higher than those in control group,while hemoglobin(Hb),albumin(ALB)and estimated glo-merular filtration rate(eGFR)were lower than those of control group(all P＜0.05).The urinary albu-min/creatinine ratio(ACR),Scr,BUN,Cys-C,urinary β2-microglobulin(β2-MG)and LVMI in LVH group were higher than those in non-LVH group,and Hb,ALB,eGFR were lower than those in non-LVH group,the difference was statistically significant(all P＜0.05).The circulating expressions of ln-cRNA ANRIL in DKD patients were significantly higher than that in control group(P＜0.05).lncRNA ANRIL was positively correlated with Scr,BUN and negatively correlated with eGFR(all P＜0.05).ln-cRNA ANRIL expressions in LVH patients were dramatically higher than non-LVH group(P＜0.05).ANRIL was positively correlated with LVMI(P＜0.05),and ANRIL was an independent risk factor for LVH in DKD patients(OR=1.535,95％CI:1.058-2.225,P=0.024).ALB was a protective factor for LVH in DKD patients(OR=0.85,95％CI:0.737-0.987,P=0.033).The area under the curve of ln-cRNA ANRIL in predicting LVH in DKD patients was 0.795,the sensitivity was 81.4％and the speci-ficity was 73.9％.Conclusions lncRNA ANRIL in peripheral blood can be used as a biomarker of kid-ney damage in DKD and as a predictor of LVH assessment in DKD patients.
NETL
NSTL
万方数据
维普
