Correlation between sperm DNA fragmentation index,sperm morphology and sperm motility parameters in single center male semen samples
Objective To investigate the correlation between sperm DNA fragmentation index(DFI)and sperm morphology and sperm motility parameters.Methods Semen samples from 475 male patients treated in our hospital from May 2020 to July 2021 were selected.According to DIF,the patients were divided into 3 groups:DFI＜15％group(178 cases),15％≤DFI≤30％group(155 ca-ses)and DFI＞30％group(142 cases).Sperm morphology and sperm movement parameters of the three groups were compared.Pearson correlation was used to analyze the correlation between DFI and semen rou-tine indexes,sperm morphology and sperm motility parameters.Results In DFI＜15％group,there were statistically significant differences in progressive motility(PR),non-progressive motility(NP),PR+NP,forward motile sperm concentration,non-forward motile sperm concentration,total forward motile sperm,non-forward motile sperm total greater than 15％≤ DFI ≤30％group and DFI＞30％group(all P＜0.001).The immobile aperm rate(IM),motionless sperm concentration and total motionless sperm in DFI＞30％group were higher than those in DFI＜15％group and 15％≤DFI≤30％group,and the differences were statistically significant(all P＜0.001).There were statistically significant differences in normal sperm morphology rate,curve velocity of sperm movement,linear velocity of sperm movement,path velocity,and lateral head swing amplitude in DFI＜15％≤DFI≤30％group and DFI＞30％group(all P＜0.001).There was no significant difference in motion oscillation among the three groups(P＞0.05).The straightness,forward-orientation and whipping frequency of DFI＞30％group were higher than those of DFI＜15％group and 15％≤DFI≤30％group,and the differences were statistically sig-nificant(all P＜0.001).Sperm DFI was positively correlated with the rate of immobile sperm,concen-tration of immobile sperm and total number of immobile sperm,and negatively correlated with PR,NP,PR+NP,concentration of forward motile sperm,concentration of non-forward motile sperm,total num-ber of forward motile sperm and total number of non-forward motile sperm(P＜0.05).DFI was nega-tively correlated with normal sperm morphology rate(P＜0.05).DIF was positively correlated with sperm straightness,forward orientation and whiplash frequency,and negatively correlated with sperm curve velocity,straight velocity,path velocity and lateral swing amplitude(all P＜0.05).Conclusions Sperm DFI is correlated with some semen routine parameters,normal morphology rate of sperm and sperm motility parameters,and can be used as a reference index of sperm quality.
万方数据
维普
