Effect of combination preconditioning on TNF-α,p38MAPK protein expression in renal ische-mia-reperfusion injury of rats
Objective To investigate the protective of AntiTNF-αcombined with p38MAPK an-tisense oligonucleotide on renal ischemia-reperfusion injuru in rats.Methods A total of 120 SD rats were randomly divided into 4 groups:Sham operation group(Sham group),ischemia reperfusion group(IR group),ischemia reperfusion+anti-TNF-α mab group(anti-TNF-A+IR group),and ischemia reperfusion+anti-TNF-α mab combined with p38MAPK antisense oligonucleotide group(combined pretreatment group),with 30 rats in each group.In Sham group,renal artery was separated but not clamped after anesthesia.When the renal ischemia reperfusion model was made in IR group,0.2 mL normal saline was injected through the tail vein 5 min before the renal blood supply was restored,and negative control was performed.The combined pretreatment group was injected with anti-TNF-α mono-clonal antibody(0.1 mg/kg,diluted with normal saline to 0.1 mL)and p38MAPK antisense oligonu-cleotide(5 mg/kg,diluted with normal saline to 0.1 mL)5 min before blood supply was restored.Anti-TNF-α+IR group was injected with anti-TNF-α monoclonal antibody(0.1 mg/kg)5 min be-fore renal reperfusion.Histopathological changes of kidney were observed by HE staining and light mi-croscopy.Two-step inmunohistochemical methods were used to detect the changes of expression of TNF-α,p38MAPK.Results Pathological damage of kidney tissue was significantly alleviated in com-bination preconditioning group.TNF-αmainly expressed in renal proximal convoluted tubules,gradually upregulate with duration of ischemia-reperfusion to 12 h of reperfusion.p38MAPK mainly located at distal convoluted tubules,peaked at 6 h of reperfusion.But these effects were offset by administration in combination preconditioning group(P＜0.05).Conclusions Renal ischemia-reperfusion injury can be alleviated by anti-TNF-α and p38MAPKantisense oligonucleotide treatment.
Reperfusion InjuryRats,Sprague-DawleyTumor Necrosis Factor-alphap38 Mi-togen-Activated Protein Kinase
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