Construction of BLCA molecular subtype and prognosis model based on inflammatory response related genes
Objective To construct BLCA molecular subtypes and prognostic Molecular signa-ture through the expression data and clinical data of inflammatory response related genes(IRRGS)in bladder urothelial carcinoma(BLCA)in TCGA database,and to explore its prognostic role in BLCA.Methods Firstly,the transcriptome data and clinical prognosis data of 412 patients with BLCA in TCGA database were downloaded.The expression of BLCA IRRGS was analyzed by unsupervised clus-ter analysis using NMF package in R language,and BLCA molecular subtypes based on IRRGS were constructed to explore the prognosis of different subtypes and tumor microenvironment.LASSO-Cox re-gression was used to screen the methylation markers related to the prognosis of BLCA,and construct the prognostic molecular tag to further explore its prognostic role in BLCA.Results Based on BLCA transcriptome data and survival data,33 of the 200 IRRGS were found to be related to the prognosis of BLCA(all P＜0.05).BLCA molecular subtypes were constructed based on 33 IRRGS.Four hundred and twelve patients with BLCA were divided into three subgroups.Different subtypes had different prognosis,and different subtypes had different tumor microenvironment.Based on 33 IRRGS,nine IR-RGS(DCBLD2,IL10,IRAK2,IRF1,LDLR,PVR,RIPK2,SEMA4D and TLR2)were selected by LASSO-Cox regression analysis.Combined with 9 IRRGS,prognostic molecular signature was con-structed.Molecular signature could well judge the prognosis of BLCA patients.Conclusions BLCA molecular subtypes are constructed based on IRRGS,and the prognosis is different among different subtypes.The tumor microenvironment is different among different subtypes.BLCA prognosis Molecu-lar signature based on 9 IRRGS can well judge the prognosis of BLCA.These results suggest that IR-RGS may play an important role in BLCA.
InflammationGenesUrothelial Carcinoma of the Urinary BladderMolecular Sig-nature
NETL
NSTL
万方数据
