Clinical significance of peripheral blood B lymphocyte detection in children with hormone de-pendent and frequent recurrent nephrotic syndrome
Objective To investigate the clinical significance of peripheral blood B lympho-cyte detection in children with hormone-dependent and frequently relapsed nephrotic syndrome(SDNS/FRNS).Methods The clinical data of 40 children with SDNS/FRNS who received initial glucocorti-coid(GC)treatment in our hospital from January 2022 to January 2023 were retrospectively analyzed,and they were set as the study group.At the same time,40 children with infrequent recurrent nephrotic syndrome who received initial GC treatment were selected as the control group.The absolute number of NK cells before and after treatment was compared between the two groups,and the expression level of B lymphocyte subsets before and after treatment was compared.Meanwhile,the relationship between CD19+B lymphocytes and their subsets and the absolute number of NK cells in the study group was an-alyzed.Results Active period.The absolute number of memory B cells,transitional B cells and total CD19+B cells in the study group were higher than those in the control group,and the difference was significant(all P＜0.05).The absolute number of NK cells in the study group was significantly lower than that in the control group(P＜0.05).Remission period.There was no significant difference in the absolute number of plasma cells,initial B cells,transitional B cells,memory B cells,NK cells and total CD19+B cells between the two groups(all P＞0.05).The total CD19+B cell absolute num-ber,memory B cell absolute number and transitional B cell absolute number in the remission stage were lower than those in the active stage,the difference was significant(all P＜0.05).There was no significant difference in the absolute number of plasma cells and initial B cells between the two groups(all P＞0.05).The absolute number of NK cells in the remission stage was higher than that in the ac-tive stage,and the difference was significant(P＜0.05).In the study group,the absolute number of NK cells was not correlated with the absolute number of plasma blasts,the absolute number of initial B cells(all P＞0.05),but with the absolute number of memory B cells,and the absolute number of transitional B cells(P＜0.01),the absolute number of CD19+B cells(r=-0.553,P＜0.01)showed a significant negative correlation.Conclusions The phenotypic distribution of peripheral blood B lymphocytes in children with SDNS/FRNS and the absolute number of memory B cells and transition-al B cells are abnormal,and both transitional B cells and memory B cells may have clearance barriers,and NK cells may be involved in the clearance process.
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