Effects of microRNA-505 on malignant biological behavior of prostate cancer cells by regulating PLK1 and its mechanism
Objective To investigate the effects of miR-505 on the proliferation,migration and invasion of prostate cancer PC-3 cells by regulating the expression of Polo-like kinase 1(PLK1).Methods PC-3 cells in the fourth generation of logarithmic growth phase were randomly divided into control group,NC group,miR-505 inhibitor group,mimic NC group and miR-505 mimic group.Ex-cept the control group,cells in the other groups were transfected with inhibitor NC,miR-505 inhibitor,mimic NC and miR-505 mimic,respectively.The control group was not treated.After 48 h,qRT-PCR was used to detect the expression of miR-505,MTT assay was used to detect cell survival rate,scratch assay was used to detect cell migration ability,Transwell assay was used to detect cell invasion ability,dual luciferase reporter gene was used to detect the targeting relationship between miR-505 and PLK1,and western blot was used to detect the relative expression levels of PLK1,Vimentin,neurocadherin(N-cad)and epithelial cadherin(E-cad)proteins.Results Compared with the control group,the relative expression of miR-505 and E-cad protein were decreased in miR-505 inhibitor group,while cell survival rate,cell migration rate,and the relative expression of PLK1,Vimentin and N-cad pro-tein were increased,and the cell invasion ability was enhanced(all P＜0.05).The relative expres-sion of miR-505 and E-cad protein increased in miR-505 mimic group,while cell survival rate,cell migration rate,the relative expression of PLK1,Vimentin and N-cad protein decreased(all P＜0.05).Compared with NC group,the relative expression of miR-505 and E-cad protein in miR-505 inhibitor group were decreased,while cell survival rate,cell migration rate,the relative expression of PLK1,Vimentin and N-cad protein in miR-505 inhibitor group were increased,and the cell invasion ability was enhanced(all P＜0.05).Compared with miR-505 inhibitor group,the relative expression of miR-505 and E-cad protein in miR-505 mimic group increased,while cell survival rate,cell migra-tion rate,the relative expression of PLK1,Vimentin and N-cad protein in miR-505 mimic group de-creased,the cell invasion ability was decreased(all P＜0.05).Compared with NC mimic group,the relative expression of miR-505 and E-cad protein in miR-505 mimic group increased,while cell surviv-al rate,cell migration rate,the relative expression of PLK1,Vimentin and N-cad protein in miR-505 mimic group decreased(all P＜0.05).Conclusions miR-505 attenuates the proliferation,migration and invasion of prostate cancer cells,possibly by inhibiting the expression of PLK1.
Prostatic NeoplasmsPolo Like Kinase 1miR-505Cell ProliferationCell Move-ment
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