Application of high-throughput second-generation gene sequencing technology in diagnosis and treatment of pulmonary infection after renal transplantation
Objective To explore the effect of high-throughput second-generation gene se-quencing(mNGS)in diagnosis and treatment of pulmonary infection after renal transplantation.Meth-ods The medical history data of 70 kidney transplant recipients with pulmonary infection who received treatment at the Kidney Transplant Center of Chenzhou First People's Hospital from January 2018 to July 2022 were retrospectively analyzed.The patients were divided into mNGS group(32 cases)and traditional group(38 cases)according to whether alveolar lavage fluid(BALF)mNGS test was used.Pathogen detection methods in the mNGS group include conventional detection and BALF mNGS detec-tion.Among them,traditional tests include specimen culture,experimental detection of fungal D glu-can,detection of Aspergillus galactommannan,cytomegalovirus DNA,and serological test of Myco-plasma pneumoniae.Clinical data such as positive rate of etiological detection,length of stay and fatal-ity rate were compared between the two groups.Results The positive rate of mNGS detection in the mNGS group was significantly higher than that in the traditional detection method and the traditional group,and the differences were statistically significant(all P＜0.05).The detection rate of mixed in-fection in mNGS detection method was higher than that of traditional detection method,and the differ-ence was statistically significant(P＜0.05).After treatment,two patients(5.26％)died in the tra-ditional group and none in the mNGS group.In addition,the length of hospitalization in the traditional group and the mNGS group was(17.22±8.62)d and(12.58±10.08)d,respectively,and the difference was statistically significant(P＜0.05).Conclusions In the diagnosis and treatment of lung infection after kidney transplantation,mNGS detection can increase the positive rate of pathogens,reduce the length of hospital stay and mortality,and has important value in improving the prognosis and economic benefits of recipients after kidney transplantation.
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