Effects of Perilla leaf extract on renal fibrosis and Notch/Snail1 signaling pathway in diabetic kidney disease rats
Objective To analyze the effects of Perilla leaf extract on renal fibrosis and Notch/zinc finger protein transcription factor 1(Snail1)signaling pathway in diabetic kidney disease rats.Methods 40 rats were selected,10 were used as blank group,and the remaining 30 rats were established with diabetic kidney disease model.A total of 29 rats were successfully modeled,and they were divided into model group 9,metformin group 10,and Perilla leaf extract group 10.The metformin group was given,the Perilla leaf extract group was given,the blank group and the model group were not treated,and the changes were ob-served after 14 days of intervention.Results Compared to the blank group,Levels of blood glucose,urea nitrogen(BUN),creatinine(Scr),alanine aminotransferase(ALT),aspartate aminotransferase(AST),type Ⅳ collagen(Ⅳ-C),type Ⅲ procollagen(PCⅢ),hyaluronic acid(HA),laminin(LN),Notch and Snail1 in model group,metformin group and Perilla leaf extract group mRNA expression level and relative expression level were increased(P＜0.05).Compared with model group,the levels of blood glucose,BUN,SCr,ALT,AST,Ⅳ-C,PCⅢ,HA,LN,Notch and Snail1 mRNA expression and relative expression of metformin group and Perilla leaf extract group were decreased(P＜0.05).The mRNA expression levels of blood glucose,BUN,SCr,ALT,AST,Ⅳ-C,PCⅢ,HA,LN,Notch and Snail1 and their relative expres-sion levels were significantly different between the metformin group and Perilla leaf extract group(P＞0.05).Conclusion After treatment with Perilla leaf extract,renal fibrosis was alleviated and Notch/Snail1 signa-ling pathway was inhibited.
Perilla leaf extractStreptozotocinDiabetic nephropathyRenal fibrosisNotch/Zinc finger protein transcription factor 1
NETL
NSTL
万方数据
