Based on bioinformatics analysis and experimental verification,the composition and mechanism of TCM anti-diabetic nephropathy were discussed
Objective To explore the medication rules and mechanism of anti-diabetic nephropathy(DKD)in traditional Chinese medicine,and verify it through animal experiments.Methods We collected the literature reports on anti-DKD in TCM,extracted the information of formulas,screened the high-confi-dence and supportive drug combinations as new formulas,and obtained the active ingredients and targets of the new formulas;we obtained the DKD-related datasets GSE30122 and GSE30529 from the GEO database,and identified the new formulas'anti-DKD hub genes by combining the Weighted Gene Co-Expression Net-work(WGCNA)and machine learning.genes and validate the expression differences of hub genes.Results The combination of"Astragalus-Salvia-Comus officinalis"as a new formula involves 1,378 targets.Based on GSE30122 and GSE529,19 targets were further obtained,and the targets were mainly enriched in the Hippo signaling pathway,cGMP-PKG signaling pathway,etc.and finally,we screened out two hub genes:EDNRA and HDAC9.EDNRA and HDAC9.Compared with the control group,the expression of Hub Genes was higher in the model group,and the renal tissue damage was more severe,and the expression of Hub Genes was effectively reduced in the treatment group after the intervention of Xinfang,and the renal tis-sue was improved compared with the model group.Conclusion As a new formula,"Astragalus-Salvia-Cor-nus officinalis"may inhibit kidney injury by regulating Hippo,cGMP-PKG,and other signaling pathways through EDNRA and HDAC9,thereby exerting the therapeutic effect of DKD and providing a theoretical ba-sis for the subsequent drug development and clinical application of DKD.
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