Application of multi-tissue paraffin blocks in positive controls for immunohistochemical staining
Objective:Currently,there are various methods for producing positive controls for immunohistochemical staining.Single-tissue controls are simple to make,but their use can lead to errors due to the multitude of immunohistochemical staining indicators.Some multi-tissue controls are convenient to use but are complex and difficult to produce.This study aims to explore a novel and simple method for creating multi-tissue paraffin blocks and their application in immunohistochemical staining controls.Methods:Based on the recommendations of the International Ad Hoc Expert Committee for common antibody control tissues,paraffin blocks containing liver,appendix,tonsil,lung,and pancreas tissues were selected,with one block from each tissue type forming a group.Ten groups were prepared,and continuous sections of 6 μm thickness were cut.The tissue sections were then rolled and stacked together to form a long,dense tissue roll,which was subsequently cut and embedded to create multi-tissue immunohistochemical staining positive control paraffin blocks.These blocks were then used in immunohistochemical staining.Results:The positive control paraffin blocks demonstrated good staining effects,containing 5 types of positive control tissues and suitable for 68 commonly used immunohistochemical staining markers.At least 2 control paraffin blocks were made at a time,taking approximately 20 minutes.The average tissue size was about 4.7 mm×4.1 mm.The immunohistochemical staining results were consistent between different control blocks and different sections of the same control block.On average,one paraffin block yielded approximately 800 effective immunohistochemical staining sections.Conclusion:The multi-tissue positive control paraffin blocks are simple to produce,time-efficient,have small tissue areas,good consistency,and high repeatability.They can meet the needs for setting up controls for 68 commonly used antibodies and have good clinical application value for routine immunohistochemical staining.
国家科技期刊平台
NSTL
万方数据
