Detection of content and stability of short-chain fatty acids in feces
Objective:Short-chain fatty acids(SCFAs)are important metabolites of the gut microbiota,closely related to metabolic diseases,and serve as significant signaling molecules in the holistic regulation of metabolic diseases in traditional Chinese medicine.This study investigates the impact of different storage conditions on the content of SCFAs in feces to provide reference for SCFA detection related to metabolic diseases.Methods:Gas chromatography was used to detect SCFAs in fecal samples and processed fecal samples under various storage conditions(room temperature,-20℃,-80℃)and times(0,2,4,6,8 hours,or 1,3,7,15,30 days).The relative standard deviation(RSD)was used to evaluate the stability of SCFA content in fecal samples under different storage temperature.Results:The RSD values of SCFA content in processed fecal samples were consistently lower than those in original fecal samples across different temperatures.SCFA content RSD values for samples stored at-80℃were lower than those at-20℃.For processed fecal samples the RSD values of SCFA content showed minimal variation across different storage temperatures.SCFA content in original fecal samples stored at-20℃showed no significant change within 15 days,and no significant change was observed within 30 days at-80℃.Conclusion:The stability of SCFA content in processed fecal samples is superior to that in original fecal samples.Fecal samples should be processed promptly after collection.If immediate processing is not possible,samples can be stored at-20℃or-80℃,where SCFA content remains stable for up to 15 days at-20℃or 30 days at-80℃.
万方数据
维普
