利用恒河婴猴模型对肠道病毒71型传播感染特征的生物学分析
Biological characteristics related to the transmission of enterovirus 71 in neonatal rhesus monkeys
王晶晶 1李薇 2赵红玲 2刘龙丁 2唐东红 2杨丽仙 2董承红 2纳锐雄 2王丽春 2张莹 2梁燕 2廖芸 2李琦涵2
作者信息
- 1. 中国医学科学院/北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明,650118;昆明医学院,昆明,650032
- 2. 中国医学科学院/北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明,650118
- 折叠
摘要
本文在前期工作基础上,进一步对肠道病毒71型(EV71)从恒河婴猴的感染个体向其他未感染个体传播的可能性及相关生物学特性做了初步分析.通过喷雾形式经呼吸道感染1~2月龄恒河婴猴(A组);在观察临床症状同时,于感染后第7天,取该组动物粪便处理后,将上清液以喷雾形式经呼吸道感染新的婴猴个体(B组),随后对该次代感染个体进行同样临床观察;并对哺乳B组婴猴的母猴(C组)也进行全面观察和分析.同时,将A组动物粪便处理后的上清液经血液感染的婴猴作为对照.分析临床症状,血液、粪便及咽拭子病毒载量,中和抗体效价,组织病理学改变.结果提示,恒河婴猴作为EV71感染动物模型,能反映病毒在个体间传播的基本生物学特征.依据实验结果,可以初步推断,EV71在易感人群中的主要传播途径是呼吸道;同时,病毒也可从低龄个体向成年个体传播.这些资料为进一步探讨EV71在儿童群体中的发病规律提供了一定的理论依据.
Abstract
Using an established animal model of enterovirus 71 (EV71) infection, the risk of EV71 transmission from infected neonatal to uninfected rhesus monkeys was assessed. Two 1-2 month-old neonatal monkeys were infected with EV71 via nasal spray (Group A). At 7 days post-infection, two neonatal monkeys assigned to Group B were inoculated with extracts from the feces of monkeys from Group A, via the same route. Meanwhile, two neonatal monkeys were inoculated with fecal extracts via intravenous injection (control group). The mothers of Group B were also enrolled as Group C. Maternal and infant data including clinical symptoms, viral load monitoring, and neutralizing antibody titers were obtained every day post-infection. The results from virological and pathological analysis showed that the transmission of EV71 could be observed in the neonatal monkey model. These findings also showed that the transmission of EV71 was more likely via respiratory route and that infection could be passed from infant to mother. These results provide additional information on the search for factors related to EV71 infection in childhood.
关键词
肠道病毒71型/恒河猴/传播/感染/动物模型Key words
Enterovirus 71/Rhesus monkey/Transmission/Infection/Animal model引用本文复制引用
基金项目
国家科技重大专项项目(2008ZX10004-014)
国家重点基础研究发展规划(973计划)(2011CB04903)
出版年
2011
NETL
NSTL
维普
万方数据