上海市2014-2019年麻疹疑似病例实验室监测结果及病原谱分析
Pathogen spectrum analysis in suspected measles cases in Shanghai from 2014 to 2019
崔晓娴 1周艳秋 1王煜垟 1崔心怡 1李智 1陈敏 1张曦1
作者信息
- 1. 上海市疾病预防控制中心病原生物检定所,上海 200036
- 折叠
摘要
本文旨在探究上海市麻疹疑似病例的病原谱构成.从2014-2019年的3 325份麻疹/风疹阴性咽拭子标本中,挑选1 197份标本,利用荧光定量PCR技术检测水痘带状疱疹病毒(VZV)、人细小病毒B19、人类庖疹病毒4型(Epstein Barr virus,EBV)、人单纯疱疹病毒1型(HSV-1)、人疱疹病毒6型(HHV-6)、人疱疹病毒7型(HHV-7)、柯萨奇病毒A16(CA16)、柯萨奇病毒A6型(CA6)、柯萨奇病毒A10型(CA10)、肠道病毒71型(EV71)及其它通用肠道病毒(EV).结果显示,共检测到9种病毒,总检出率为9.7％,未检出混合感染.EBV检出率最高,为1.8％,其次为人疱疹病毒6型(1.7％)、VZV及人细小病毒B19(各1.3％).其他EV检出率为0.8％;检出CA6、CA10各7例(0.6％);检出HSV-1共6例(0.5％),CA16有4例(0.3％).人细小病毒B19、VZV主要在未成年人中检出,EBV、HHV-6、CA16、CA6在成人和未成年人中占比相似.VZV、EBV及HHV-6主要在男性中检出,HSV-1主要在女性中检出.本研究提示,在上海市麻疹疑似病例中,除麻疹病毒、风疹病毒外,还存在EB病毒、HSV-1、细小病毒B19等9种病毒,它们均可能引起类似麻疹症状而被临床诊断为麻疹,临床医师诊断时须注意加以鉴别.
Abstract
To explore the pathogen spectrum in suspected measles cases in Shanghai from 2014 to 2019,1 197 pharyngeal swab specimens were collected from 3 325 measles/rubella negative speciments and screened to detect eleven rash fever associated viruses,including varicella-zoster virus,human parvovirus B19,HSV-1,HHV-6,HHV-7,EBV and other enteroviruses by real time PCR.A total of 9 viruses were detected,and the virus detection rate was 9.7％(108/1 197).Mixed infection was not detected.The positive rate of human Epstein-Barr virus was the highest(1.8％),followed by HHV-6(1.7％),varicella-zoster virus and human parvovirus B19(1.3％respectively).The positive rate of other EV was 0.8％.CA6 and CA10 were found in 7 cases with a positive rate of 0.6％,respectively.HSV-1 was found in 6 cases,and the positive rate was 0.5％.4 cases were CA16,and the positive rate was 0.3％.Human parvovirus B19 and VZV were mainly detected in children.EB,HHV-6,CA16 and CA6 were detected in adults and children at similar rates.Human parvovirus B19 and other enteroviruses were mainly detected in adult cases.VZV,EBV and HHV-6 were mainly detected in males,and HSV-1 was mainly detected in females.In addition to measles and rubella,9 viruses including EBV,HSV-1 and human parvovirus B19,may cause fever and rash symptoms and be clinically diagnosed as measles.More attention should be paid to the clinical diagnosis.
关键词
麻疹/疑似病例/发热出疹疾病/病原谱/聚合酶链式反应Key words
Measles/Suspected cases/Rash and fever illness/Pathogen spectrum/PCR引用本文复制引用
基金项目
国家重点研发计划(2023YFC3041500)
上海市卫生健康委员会青年课题(20204Y1087)
出版年
2024
NETL
NSTL
万方数据