Expression of miR-29 in patients with hepatitis B cirrhosis and its effect on activation of hepatic stellate cells and epithelial mesenchymal transition
Objective This paper is to investigate the expression of miR-29 in patients with hepatitis B cirrhosis and its effect on the activation of hepatic stellate cells(HSC)and epithelial mesenchymal transition(EMT).Methods A total of 104 patients with hepatitis B admitted to Yingcheng People's Hospital from June 2020 to June 2022 were selected,including 52 patients with simple hepatitis B(set as the chronic hepatitis B group)and 52 patients with hepatitis B complicated with cirrhosis(set as the hepatitis B cirrhosis group).The expression of serum miR-29a,miR-29b,and miR-29c was detected by real-time fluorescence quantitative PCR,to analyze the relationship between miR-29 and Child-Pugh grading and clinical staging in patients with hepatitis B cirrhosis.Using miR-29a/b/c recombinant lentivirus to infect human HSC line LX-2,an overexpression miR-29 cell model was prepared,and the effects of the overexpression miR-29 on LX-2 cell proliferation,activation,and EMT were analyzed.Results Compared with the chronic hepatitis B group,the expression of serum miR-29a,miR-29b,and miR-29c in the hepatitis B cirrhosis group is lower,with statistically significant differences(P＜0.01).The expression of serum miR-29a,miR-29b,and miR-29c in patients with hepatitis B cirrhosis is gradually decreased with the increase of Child-Pugh grade and clinical stage,with statistically significant differences(P＜0.01).Pearson correlation analysis shows that the expression of serum miR-29a,miR-29b,and miR-29c in patients with hepatitis B cirrhosis is significantly negatively correlated with Child-Pugh grade and clinical stage(P＜0.01).Compared with the blank control group,the cell proliferation rate of the recombinant lentivirus group is reduced,and the expression of α-smooth muscle actin(α-SMA),type Ⅰ collagen(COLⅠ),type Ⅲ collagen(COLⅢ),transforming growth factor-β1(TGF-β1),Vimentin,N-cadherin protein,and mRNA is decreased,while the expression of E-cadherin protein and mRNA is increased,with statistically significant differences(P＜0.01).Conclusions The expression of serum miR-29 in patients with hepatitis B cirrhosis is low,and is related to Child-Pugh grade and clinical stage.Overexpression of miR-29 may play an anti-hepatic fibrosis role by inhibiting the proliferation,activation of HSC and EMT.
Hepatitis B cirrhosismiR-29Liver fibrosisHepatic stellate cellsEpithelial mesenchymal transformation
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