Objective This paper attempts to investigate the expression of miR-802 in hepatocellular carcinoma cells and its effect on tumor angiogenesis and mechanism of action.Methods The expression of miR-802 in human hepatocellular carcinoma cell line SMMC-7721 was up-regulated or inhibited by transfection of miR-802 agomir and miR-802 antagomir.SMMC-7721 cells were divided into the NC group(normal cultured cells),the miR-802 agomir group(transfected with miR-802 agomir),and the miR-802 antagomir group(transfected with miR-802 antagomir).The effects of SMMC-7721 cell supernatant overexpressing miR-802 on the tube-forming and migratory abilities of human umbilical vein endothelial cells(HUVEC)were detected by using tubule formation assay and Transwell assay.The expression of phosphatidylinositol 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),phosphorylated Akt(p-Akt),and vascular endothelial growth factor(VEGF)was detected by western blotting in the miR-802 agomir group.Immunofluorescence assay was used to detect the effect of overexpression of miR-802 on VEGF production.Results Compared with the NC group,the tube-forming and migratory abilities of HUVEC cells in the miR-802 agomir group are enhanced,and the tube-forming and migratory abilities of HUVEC cells in the miR-802 antagomir group are reduced,with statistically significant differences(P＜0.05).Compared with the NC group,the production of VEGF of SMMC-7721 cells in the miR-802 agomir group is increased,and the protein expression of p-PI3K,p-Akt,and Akt is elevated,with statistically significant differences(P＜0.05).Conclusion miR-802 can promote the release of VEGF through regulating the PI3K/Akt signaling pathway,and then participate in angiogenesis,thus promoting the occurrence and progression of hepatocellular carcinoma.
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