Objective This paper aims to analyze the expression of cyclin dependent kinase 14(CDK14)and folate binding protein 1(FOLR1)in colon cancer and their relationship with clinicopathological characteristics and prognosis of patients.Methods A hundred and seventy-seven colon cancer patients admitted to Honghu People's Hospital from October 2016 to October 2019 were selected as the research subjects,and their colon cancer tissues and adjacent tissues were collected after surgical resection.Real time fluorescence quantitative PCR(qRT-PCR)was used to detect the mRNA expression levels of CDK14 and FOLR1 in tissues while the immunohistochemical method was used to detect the protein expression of CDK14 and FOLR1 in tissues.The Pearson method was used to analyze the correlation between CDK14 and FOLR1 expression in colon cancer tissues.The Kaplan Meier survival curve was used to analyze the relationship between CDK14 and FOLR1 expression in colon cancer tissues and patient prognosis(using Log-rank test).The COX regression analysis was used to explore the risk factors for prognosis in colon cancer patients.Results The mRNA expression levels and protein expression positivity rates of CDK14 and FOLR1 in colon cancer tissues are significantly higher than those in adjacent tissues(P＜0.05).The Pearson correlation analysis results show a positive correlation between CDK14 and FOLR1 expression in colon cancer tissues(P＜0.05).The expression of CDK14 and FOLR1 in colon cancer tissue is correlated with TNM staging,differentiation degree,and infiltration depth of patients(P＜0.05).The Kaplan-Meier survival curve analysis results show that the 3-year cumulative survival rates of patients with CDK14 and FOLR1 positive expression in colon cancer tissue are significantly lower than those of patients with CDK14 and FOLR1 negative expression,respectively(P＜0.05).The results of COX regression analysis show that the positive expression of CDK14 and FOLR1 in colon cancer tissues are risk factors for the prognosis of colon cancer patients(P＜0.05).Conclusion The expression levels of CDK14 and FOLR1 are significantly increased in colon cancer tissues,and both are closely related to the TNM staging,differentiation degree,infiltration depth,and prognosis of patients,and the positive expression of CDK14 and FOLR1 are risk factors for the prognosis of colon cancer patients.
Cyclin dependent kinase 14Folate binding protein 1Colon cancerClinicopathological characteristicsPrognosis
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