Objective This paper intends to investigate the expression of diaphanous related formin 3(DIAPH3)in pancreatic carcinoma tissues and its prognostic prediction value.Methods A total of 68 patients with pancreatic cancer who underwent radical surgery in the Affiliated Huai'an Hospital of Xuzhou Medical University from March 2017 to March 2022 were selected in the study,and the fresh pancreatic carcinoma tissues and paracancerous tissues(＞5 cm away from the tumor tissue margin)were collected.The expression of DIAPH3 in pancreatic carcinoma tissues was detected by immunohistochemical staining(SP method),and the relationship between the expression of DIAPH3 in pancreatic carcinoma tissues and the clinicopathological features of patients was analyzed.The Cox proportional hazards regression model was used to analyze the prognostic factors of pancreatic cancer patients.All patients were followed up for one year from the day of operation.A survival curve was plotted by using the Kaplan-Meier method and a Log-rank test was conducted to compare the 1-year survival rate in patients with DIAPH3 expression in pancreatic cancer.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of DIAPH3 on the one-year survival rate of pancreatic cancer patients.Results The positive expression rate of DIAPH3 in pancreatic carcinoma tissues is significantly higher than that in paracancerous tissues(55.88%versus 14.71%),with a statistically significant difference(χ2=25.242 and P=0.000).The positive expression rate of DIAPH3 in pancreatic carcinoma tissues with tumor maximum diameter≥3 cm,lymph node metastasis,poor differentiation,TNM stage Ⅲ-Ⅳ,tumor located in the body/tail of the pancreas,CA19-9＞37 kU/L are higher than those with tumor maximum diameter＜3 cm,no lymph node metastasis,medium and high differentiation,TNM stage Ⅰ-Ⅱ,tumor located in the head of the pancreas,CA19-9≤37 kU/L,with statistically significant differences(P＜0.05).The multivariate Cox regression model analysis shows that the largest tumor diameter,differentiation degree,CA19-9,DIAPH3 are independent risk factors for one-year survival rate of patients with pancreatic cancer(P＜0.05).The one-year survival rate of the DIAPH3 positive expression group is lower than that of the negative expression group(55.26%versus 80.00%),and the average survival time is shorter than that of the negative expression group(164.32±18.53 d versus 241.54±19.28 d),with statistically significant differences(P＜0.05).The ROC curve analysis indicates that the area under the curve(AUC)of DIAPH3 in pancreatic carcinoma tissues predicting one-year survival rate of patients is 0.768(95%CI:0.612-0.935),the sensitivity is 82.95%,and the specificity is 63.21%.Conclusion DIAPH3 is highly expressed in pancreatic carcinoma tissue,which may participate in the occurrence and progression of pancreatic cancer and become an important reference index to predict the one-year survival rate of patients after surgery.
Pancreatic cancerDiaphanous related formin 3Clinicopathological featuresPrognosis
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