Objective This paper aims to investigate the expression of serpin peptidase inhibitor clade E member1(SERPINE1)in gastric cancer and its mechanism of action on immune escape and β-catenin/N-cadherin signaling pathway in gastric cancer cells.Methods Seventy-eight patients who underwent gastric cancer surgery at Dingzhou People's Hospital from January 2020 to January 2022 were selected as the research subjects.Their gastric cancer tissues and adjacent tissues were collected,and the expression of SERPINE1 in the tissues was detected by immunohistochemistry.The human gastric cancer cell line SGC-7901 was divided into the control group(SGC-7901 cells),the oe-SERPINE1 group(pcDNA3.1-SERPINE1 vector),the oe-NC group(empty pcDNA3.1 vector),the SERPINE1 siRNA group(SERPINE1 siRNA),and the si-NC group(si-NC)after different treatments.The real-time fluorescence quantitative PCR was used to detect the expression level of SERPINE1 mRNA in each group of cells,the Transwell method was used to detect the number of invasions in each group of cells,the scratch assay was used to detect the migration distance of each group of cells,and the Western-blotting was used to detect the protein expression levels of programmed death receptor-1(PD-1),programmed death ligand-1(PD-L1),β-catenin,and N-cadherin in each group of cells.Results The positive expression rates of SERPINE1 in gastric cancer tissue and adjacent tissues are 88.46% and 19.23%,respectively,with a statistically significant difference(P＜0.05).The expression levels of SERPINE1 mRNA,invasion number,migration distance,as well as the expression levels of PD-1,PD-L1,β-catenin,and N-cadherin proteins in the oe-NC group cells show no statistically significant differences compared to those of the control group(P＞0.05).The above indicators of the oe-SERPINE1 group cells are significantly higher than those of the oe-NC group(P＜0.05).The above indicators of si-NC group cells are significantly lower than those of the oe-SERPINE1 group(P＜0.05).The above indicators of SERPINE1 siRNA group cells are significantly reduced compared to those of the si-NC group(P＜0.05).Conclusions SERPINE1 is highly expressed in gastric cancer tissues.Downregulation of SERPINE1 expression can significantly inhibit the invasion and migration of gastric cancer cells and improve the immune escape of tumor cells by inhibiting the expression of PD-1 and PD-L1,which is related to the inhibition of the activity of the β-catenin/N-cadherin signaling pathway.
Gastric cancerSerpin peptidase inhibitor clade E member1Immune escapeInvasionTransferβ-cateninN-cadherin
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NSTL
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