Objective This paper intends to analyze the correlation between protein/nucleic acid decarboxylase 1(DJ-1)expression in colorectal cancer(CRC)tissue and regional lymph node metastasis(LNM)as well as patient prognosis.Methods Two hundred and fifty-eight CRC patients who underwent radical surgery at Guilin People's Hospital from June 2017 to April 2022 were selected as the research subjects.CRC tissues from the patients were collected,of which 81 patients had paired adjacent cancer tissues.Another 58 patients who underwent endoscopic treatment during the same period were selected as controls for colorectal adenoma tissue.The relationship between the expression of different DJ-1 proteins in CRC tissues and the clinical pathological characteristics of patients were analyzed.The immunohistochemistry was used to analyze the expression of DJ-1 in tissues.The multivariate logistic regression analysis was used to explore the predictive factors of regional LNM.The univariate and multivariate Cox regression analyses were used to explore the factors affecting the recurrence and death of CRC patients.The Kaplan-Meier survival curves were used to analyze the 5-year recurrence free survival rate and the cumulative survival rate of patients with different DJ-1 expressions.Results There is a statistically significant difference in the expression of DJ-1 protein among the three groups(χ2=202.557 and P＜0.001).The proportion of patients with moderate and strong expression of DJ-1 protein in CRC tissues is significantly higher than that in adjacent cancer tissues and colorectal adenoma tissues(P＜0.05).The age of patients in the high expression group is relatively older,with 55.56% of patients having primary lesions located in the left colon.The proportion of poorly differentiated CRC patients is relatively high,and the positive rate of regional LNM in patients is also high(P＜0.05).The results of logistic regression multivariate analysis show that the high expression of DJ-1 protein in CRC tissues could independently predict regional LNM and poorly differentiated CRC(P＜0.05).The 5-year recurrence free survival rate and cumulative survival rate of the patients were 67.44% and 70.54%,respectively.The results of univariate and multivariate Cox regression analysis show that the high expression of DJ-1 protein in CRC tissues is an independent risk factor for recurrence and death in CRC patients(P＜0.05).The above results are basically consistent with the bioinformatics analysis results based on the Oncomine database and TCGA cohort.Conclusions The high expression of DJ-1 protein in CRC tissues is associated with the regional LNM,which may be a prognostic factor for patients.DJ-1 has the potential to become a biomarker for predicting regional LNM and prognosis in CRC patients.
NETL
NSTL
万方数据
