Objective This paper aims to investigate the effect of pachymic acid(PA)on CCl4-induced liver fibrosis in rats by regulating the JAK2/signal transducer and activator of transcription 3(STAT3)/suppressor of cytokine signaling 3(SOCS3)signal pathway.Methods Ten SD rats were randomly selected and assigned to the control group(NC group),and the remaining 43 rats were given CCl4(2 mL/kg)by gavage to build the liver fibrosis model.After modeling,three rats were randomly selected to verify successful modeling.The remaining 40 rats with liver fibrosis were randomly assigned to the liver fibrosis group,the PA group,the coumermycin A1(C-A1)group,and the PA+C-A1 group,with 10 rats in each group.Each group was intervened once a day for 7 consecutive days.ELISA was used to detect the levels of serum inflammatory factors,liver function markers,and liver fibrosis markers while H-E and Masson staining were used to observe the pathological changes and liver fibrosis of liver tissue in each group.The protein expression levels of JAK2/STAT3/SOCS3 signal pathway related proteins,α-smooth muscle actin(α-SMA),and type Ⅲ collagen(COL3)proteins in liver tissues of each group were detected using Western blotting.Results The NC group has normal cell structure,with red staining and less collagen deposition.No collagen fiber proliferation is observed,and there are no obvious lesions in the liver lobule structure and sinusoids.The sinusoids are not dilated or congested,and there is no inflammatory cell infiltration in the portal vein area.Compared with the NC group,the liver fibrosis group has disordered liver lobular structure,the liver cells are steatosis and necrosis,with inflammatory cell infiltration,liver swelling,and many small nodules on the surface.The collagen volume fraction,the levels of IL-2,IL-6,IL-17A,ALT,AST,alkaline phosphatase(ALP),hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PCⅢ),type Ⅳ collagen(Ⅳ-C),and the expression levels of α-SMA,COL3,p-JAK2/JAK2,p-STAT3/STAT3,and SOCS3 proteins are significantly increased(P＜0.05).Compared with the liver fibrosis group,the PA group shows improvement in liver injury and fibrosis,and the levels of the above indicators are significantly reduced(P＜0.05),while the C-A1 group shows significant increases(P＜0.05).Compared with the PA group,the liver cell necrosis,inflammatory cell infiltration,and degree of liver fibrosis are aggravated in the PA+C-A1 group,and the levels of the above indicators are significantly increased(P＜0.05).Conclusion PA may improve liver fibrosis in rats by inhibiting the JAK2/STAT3/SOCS3 signal pathway.
Pachymic acidJAK2Signal transducer and activator of transcription 3Suppressor of cytokine signaling 3Liver fibrosisInflammatory reaction
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