Objective This paper is to investigate the relationship between serum procalcitonin(PCT)and miR-217 and the severity of acute pancreatitis(AP),and to analyze the value of combined detection for AP prognosis.Methods One hundred and forty-seven AP patients admitted to the Fifth People's Hospital of Wuxi from January 2020 to June 2021 were selected as the study subjects and set as the AP group.According to the severity of AP disease,the patients were assigned to the mild group(33 cases),the moderate group(71 cases),and the severe group(43 cases),who were divided into the good prognosis group(85 cases)and the poor prognosis group(62 cases)based on the prognosis of the patients.Another The expression level of serum PCT in each group was detected by ELISA.The Real-time fluorescence quantitative PCR was used to detect the expression level of serum miR-217 in each group.The logistic regression model was used to analyze the risk factors affecting the poor prognosis of AP.The ROC curve was used to analyze the predictive value of serum PCT and miR-217 expression levels for poor prognosis of AP.Results Compared with the healthy group,the expression levels of serum PCT and miR-217 in the AP group are increased,with statistically significant differences(P＜0.05).Compared with the mild group,the expression levels of serum PCT and miR-217 in the moderate group and the severe group are significantly increased(P＜0.05).Compared with the moderate group,the expression levels of serum PCT and miR-217 in the severe group are significantly increased(P＜0.05).Compared with the good prognosis group,the expression levels of serum PCT and miR-217 in the poor prognosis group are significantly increased(P＜0.05).The multivariate logistic regression model analysis shows that hyperlipidemia,Acute Physiology and Chronic Health Evaluation Ⅱ(APACHEⅡ)score,pancreatic necrosis range,blood urea nitrogen(BUN),PCT,and miR-217 are independent risk factors for poor prognosis of AP(P＜0.05).The ROC curve analysis shows that the area under the curve(AUC)of the combined detection of serum PCT and miR-217 to predict poor prognosis in AP patients is 0.854,which is higher than that of the single detection,and the combined detection has the highest sensitivity.Conclusion The expression levels of serum PCT and miR-217 are closely related to the severity of AP disease,and the combined detection of both has a high predictive value for the poor prognosis of AP.
万方数据
