Serum levels of miR-22 and miR-335 in patients with ulcerative colitis and their association with disease severity and prognosis
Objective This paper aims to investigate the expression levels of serum miR-22 and miR-335 in patients with ulcerative colitis(UC)and their correlation with disease severity and prognosis.Methods A total of 115 UC patients treated at Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital from July 2019 to July 2021 were selected as the case group.Based on disease severity,the UC patients were divided into the mild UC group(n=46),the moderate UC group(n=38),and the severe UC group(n=31).According to the prognosis,they were further divided into a poor prognosis group(n=23)and a good prognosis group(n=92).Another 115 healthy individuals from the same period in the hospital were selected as the control group.Real time fluorescence quantitative PCR was used to detect the expression levels of serum miR-22 and miR-335 in each group.ROC curve analysis was performed to evaluate the diagnostic value of serum miR-22 and miR-335 for severe UC and their ability to predict poor prognosis in UC patients.Results The expression levels of serum miR-22 and miR-335 in the case group are significantly lower than those in the control group(P＜0.05).The expression levels of both serum miR-22 and miR-335 gradually decrease in the mild UC group,the moderate UC group,and the severe UC group,with statistically significant differences(P＜0.05).The ROC curve analysis reveals that the area under the curve(AUC)for serum miR-22 and miR-335 in diagnosing severe UC,both individually and combined,are 0.803,0.813,and 0.907,respectively.The combination of both biomarkers has a higher diagnostic efficacy than either biomarker alone(Z=2.015,Z=1.851,P＜0.05).The expression levels of serum miR-22 and miR-335 in the poor prognosis group are significantly lower than those in the good prognosis group(P＜0.05).The ROC curve analysis shows that the AUC for serum miR-22 and miR-335,both individually and combined,for predicting poor prognosis in UC patients are 0.794,0.807,and 0.938,respectively.Again,the combined prediction is more effective than the individual predictions(Z=2.470,Z=2.524,P＜0.05).Conclusion Serum miR-22 and miR-335 in UC patients are downregulated and have the potential to serve as biomarkers for assessing disease severity and predicting prognosis in UC patients.
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