Effects and mechanism of JPTLR in inhibiting epithelial-mesenchymal transition in colorectal cancer cells by regulating the TGF-β1/SMAD4 signaling pathway
Objective This paper attempts to investigate the effects and mechanisms of Jianpi Tongluo Recipe(JPTLR)on the proliferation,invasion,migration,and epithelial-mesenchymal transition(EMT)of colorectal cancer(CRC)cells.Methods A high-expression transforming growth factor-β(TGF-β)HCT116 cell model was established.The HCT116 cells were divided into six groups:control(NC)group,NC+TGF-β1 group,JPTLR group,JPTLR+TGF-β1 group,LY2109761 group,and LY2109761+TGF-β1 group.The scratch healing assay and Transwell invasion assay were used to evaluate cell migration and invasion.The expression levels of TGF-β1,SMAD4,and EMT-related proteins were detected by Western blotting.Results Compared to the NC group,the JPTLR group shows a significantly lower cell scratch healing rate,reduced number of invasive cells,decreased expression of SMAD4 and Vimentin,and increased expression of E-cadherin and Claudin-1,with statistically significant differences(P＜0.05).Compared to the NC+TGF-β1 group,the expression of SMAD4 and Vimentin in the JPTLR+TGF-β1 group are decreased;the expression of E-cadherin and Claudin-1 are increased,with statistically significant differences(P＜0.05).Conclusion JPTLR inhibits the proliferation,invasion,migration,and EMT of CRC cells by downregulating SMAD4 expression,increasing the expression of E-cadherin and Claudin-1,and decreasing the expression of Vimentin in HCT116 cells.
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