半夏泻心汤对2型糖尿病大鼠葡萄糖稳态的影响
Effect of the Banxia-Xiexin decoction on express of glucose homeostasis of diabetic rats
邱桂兰 1黄秀深 2张丰华2
作者信息
- 1. 610075 成都中医药大学
- 2. 610075 成都中医药大学基础医学院方剂教研室
- 折叠
摘要
目的 探讨半夏泻心汤(BD)对2型糖尿病大鼠糖原合成及垂体黑素皮质激素受体(MC4R)表达的影响以及维持葡萄糖代谢平衡的作用机制.方法 采用SD大鼠建立糖尿病模型,造模成功的大鼠按完全随机法分为糖尿病模型组、二甲双胍组(0.3 g/kg)、BD高剂量组(1.7g/kg)、BD中剂量组(0.85g/kg)、BD低剂量组(0.425 g/kg),另设正常对照组饲以正常饮食,1周后测定各组大鼠空腹体重、垂体MC4R及肝糖原的含量.结果 连续给药1周后,半夏泻心汤高、中、低剂量组大鼠的体重分别为(296.62±1.14)g、(293.83±2.17)g、(286.75±1.91)g,与糖尿病模型组(284.73±2.91)g比较,差异有统计学意义(P＜0.01)；MC4R表达分别为(29.69±1.64) ng/L、(29.38±1.88) ng/L、(30.04±1.24) ng/L,与糖尿病模型组(25.95±2.24) ng/L比较,表达均增强(P均＜0.05)；肝糖原含量分别(15.94±0.82) mg/g、(11.52±0.56) mg/g、(10.48±0.45) mg/g,与糖尿病模型组(9.05±0.44) mg/g比较含量均增加(P＜0.01、P＜0.01、P＜0.05).结论 半夏泻心汤各剂量组通过增加外周组织对糖的利用、提高MC4R表达,调节大鼠摄食行为.
Abstract
Objective To study the effects of the BanxiaXiexin decoction (BD) on express of glycogen synthesis and the expression of MC4R.and to discuss the mechanism of its maintaining glucose metabolism balance.Methods Diabetic mice were induced and divided into the model group,metformin group(0.3 g/kg)and BD high dose group(1.7 g/kg),medium dose group(0.85 g/kg),and low dose group (0.425 g/kg).In addition,a normal group was set to feed normal diet.Fasting blood glucose,body weight on an empty stomach,express of MC4R and hepatic glycogen were assayed after a week of treatment.Results Body weight of BD high,medium and low dose group was (296.62 ±1.14) g,(293.83 ± 2.1 7 ) g,and (286.75 ± 1.91 ) g respectively,showing a significant increase compared with the model group (284.73± 2.91) g (P＜0.01).MC4R expression of these MD groups were (29.69 ± 1.64) ng/L,(29.38±1.88) ng/L and (30.04 ±1.24) ng/L,showing a significant increase compared with the model group (25.95±2.24) ng/L (P＜0.05).Liver glycogen content of these MD groups were (15.94±0.82)mg/g,(11.52±0.56)mg/g,and (10.48±0.45)mg/g respectively,also showing a significant increase than the model group (9.05±0.44)mg/g(P＜0.01,P＜0.01,P＜0.05).Conclusion BD improves MC4R expression and rat feeding behavior by increasing by peripheral organization utilization of glucose.
关键词
半夏泻心汤/2型糖尿病模型/黑素皮质激素受体4/糖稳态Key words
BanxiaXiexin decoction/Type 2 diabetes mellitus/Melanin cortical hormone receptor 4/Glucose steady state引用本文复制引用
基金项目
四川省科技厅资助课题(2010JY0037)
出版年
2012
NETL
NSTL
维普
万方数据