Study on the quality of Bolbostemmatis Rhizoma standard decoction by HPLC fingerprint combined with quantitative analysis of multi-components by single marker
Study on the quality of Bolbostemmatis Rhizoma standard decoction by HPLC fingerprint combined with quantitative analysis of multi-components by single marker
Objective To establish the HPLC fingerprint of Bolbostemmatis Rhizoma standard decoction;To determine the three effective components with similar structure by quantitative analysis of multi-components by single marker(QAMS);To evaluate the quality of Bolbostemmatis Rhizoma standard decoction.Methods HPLC was adopted to establish the fingerprints of 15 batches of Bolbostemmatis Rhizoma standard decoction.The Chromatographic column was Waters XBridge Phenyl(4.6 mm×250 mm,5 μm).The mobile phase was acetonitrile-0.1％phosphoric acid solution with gradient elution.Cluster analysis(HCA)and principal component analysis(PCA)were conducted based on the relative peak area of common peaks.The same method as the fingerprint was used to establish QAMS of tubeimoside A,B,C on Bolbostemmatis Rhizoma standard decoction.Results There were 14 common peaks in the fingerprint of Bolbostemmatis Rhizoma standard decoction.It was confirmed that the peak 3 was L-tryptophan,the peak 11 was tubeimoside B,the peak 12 was tubeimoside C,and the peak 13 was tubeimoside A.15 batches of Bolbostemmatis Rhizoma standard decoction from different origins were divided into 3 categories by HCA and PCA.There was no significant difference between QAMS and the external standard method(ESM)through the system suitability inspection.Conclusion This method is accurate,reliable and has good specificity,which can effectively evaluate the quality of Bolbostemmatis Rhizoma standard decoction.
关键词
土贝母/标准汤剂/指纹图谱/土贝母苷甲/土贝母苷乙/土贝母苷丙/一测多评
Key words
Bolbostemmatis Rhizoma/Standard decoction/Fingerprint/Tubeimoside A/Tubeimoside B/Tubeimoside C/Quantitative analysis of multi-components by single marker
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