Study on identification of medicinal plant Gentiana scabra Bge.in Liaoning province based on DNA barcode sequences
Objective To identify and analyze the genuine medicinal plant Gentiana scabra Bge.from 9 regions in Liaoning Province using DNA barcode technology based on the base sequence of internal transcribed spacer.Methods DNA was extracted from the medicinal parts of 26 Gentiana scabra Bge.samples by using DNA kit extraction method.The ITS sequence was amplified through polymerase chain reaction(PCR),and then two-way sequencing was carried out.Other sources and outgroup sequences of the medicinal plant Gentiana scabra Bge.were downloaded from Genbank.After the sequencing results were spliced by using SeqMan 7.1.0 software,MEGA 7.0 software was used to analyze and compare the data,and calculate the genetic distance of K2P(Kimura 2-parameter).The phylogenetic tree was established by Neighbor-Joining(NJ)method for analysis.Results According to the results of NJ cluster tree,all Gentiana scabra Bge.samples from different sources were clustered into one large branch,and Gentiana scabra Franch.and Gentiana triflora Pall.were clustered into one branch respectively,with obvious differences;Gentiana scabra and Gentiana manshurica Kitag.were clustered into one branch,and the genetic relationship was relatively close.In combination with the variation site and genetic distance,the base sequences of Gentiana scabra and Gentiana manshurica were very similar,and the interspecific differences were very small.Except for the intraspecific variation of only one sample collected in Liaoning Province,the base sequences of the other samples were the same,and there was no difference between"Gentiana scabra Bge.in Qingyuan"and Gentiana scabra Bge.samples from other regions in Liaoning Province.Conclusion The DNA barcode technology of ITS sequence can be used to differentiate and identify medicinal plant Gentiana scabra Bge.and its original plants from different sources with a high success rate.
Gentianae Radix et RhizomaBase SequenceDNA Barcoding,TaxonomicLiaoning Province
万方数据
维普
