目的 探究针刺结合补阳还五汤对颈动脉狭窄脑血流低灌注模型大鼠肠道菌群的影响。 方法 将40只大鼠按随机数字表法分为假手术组、模型组、中药组和针药组，每组10只。除假手术组外，其余各组采用针控线栓法制备脑缺血模型。中药组灌胃补阳还五汤化裁方水煎液100 mg/kg，1次/d，连续干预2周。针药组在中药组治疗基础上，选取“百会”及其左、右侧各旁开2 mm处进行干预，1次/d，连续干预2周。分别于给药后7、14 d进行神经功能评价和行为学功能评分。采用16S rRNA测序对各组大鼠粪便菌群结构和组成进行全面表征分析，采用线性判别效应量分析法（LEfSe）对多组间肠道细菌进行差异分析。 结果 给药后第7、14天，与模型组比较，中药组和针药组神经功能评分降低（P<0.05），行为学功能评分升高（P<0.05）。与模型组比较，中药组和针药组Shannon指数升高（P<0.05）；厚壁菌门丰度升高（P<0.05），拟杆菌门和变形菌门丰度降低（P<0.05）；梭菌纲丰度升高（P<0.05），丙型变形菌纲丰度降低（P<0.05）；大肠杆菌-埃希氏菌和拟杆菌丰度降低（P<0.05）；针药组乳酸杆菌丰度显著升高（P<0.05）。 结论 针刺结合补阳还五汤治疗对颈动脉狭窄脑血流低灌注模型大鼠的症状有改善作用，其机制可能与增加益生菌丰度有关。 Objective To explore the effects of acupuncture combined with Buyang Huanwu Decoction on intestinal flora in cerebral blood flow hypo perfusion model rats with carotid artery stenosis. Methods Totally 40 rats were randomly divided into sham-operation group, model group, TCM treatment group and acupuncture and drug combination treatment group, with 10 rats in each group. Except the sham-operation group, the other groups were prepared cerebral ischemia model by needle control and thread embolism method. TCM treatment group received Buyang Huanwu Decoction 100 mg/kg for gavage, once a day, and the intervention lasted for 2 weeks. In the acupuncture and drug combination group, based on the TCM treatment group, Baihui and its left and right sides of 2 mm were selected for acupuncture, once a day, and continuous intervention was performed for 2 weeks. Neurological function evaluation and behavioral function score were performed 7 and 14 days after administration, respectively. 16S rRNA sequencing was used to comprehensively characterize the structure and composition of fecal microflora of rats in each group. Linear discriminant analysis Effect Size (LEfSe) was used to analyze the difference of intestinal bacteria among groups. Result On the 7th and 14th day after administration, compared with the model group, the neurological function score in the TCM treatment group and the acupuncture and drug combination group decreased (P<0.05), and the behavioral function score increased (P<0.05). Compared with model group, the Shannon index of TCM treatment group and acupuncture and drug combination group increased (P<0.05). The abundance of Firmicutes increased (P<0.05), and the abundance of Bacteroidetes and Proteobacteria decreased (P<0.05) the abundance of Clostridia increased (P<0.05), and the abundance of Gammaproteobacteria decreased (P<0.05). The abundance of Escherichia-Shigella and Bacteroides decreased (P<0.05) the abundance of lactobacillus significantly increased (P<0.05). Conclusion Acupuncture combined with Buyang Huanwu Decoction can improve the symptoms of cerebral hypoperfusion model rats with carotid artery stenosis, and the mechanism may be to increase the abundance of probiotics.
Effects of acupuncture combined with Buyang Huanwu Decoction on the cerebral blood flow hypo perfusion model rats with carotid artery stenosis based on high-throughput sequencing
Objective To explore the effects of acupuncture combined with Buyang Huanwu Decoction on intestinal flora in cerebral blood flow hypo perfusion model rats with carotid artery stenosis. Methods Totally 40 rats were randomly divided into sham-operation group, model group, TCM treatment group and acupuncture and drug combination treatment group, with 10 rats in each group. Except the sham-operation group, the other groups were prepared cerebral ischemia model by needle control and thread embolism method. TCM treatment group received Buyang Huanwu Decoction 100 mg/kg for gavage, once a day, and the intervention lasted for 2 weeks. In the acupuncture and drug combination group, based on the TCM treatment group, Baihui and its left and right sides of 2 mm were selected for acupuncture, once a day, and continuous intervention was performed for 2 weeks. Neurological function evaluation and behavioral function score were performed 7 and 14 days after administration, respectively. 16S rRNA sequencing was used to comprehensively characterize the structure and composition of fecal microflora of rats in each group. Linear discriminant analysis Effect Size (LEfSe) was used to analyze the difference of intestinal bacteria among groups. Result On the 7th and 14th day after administration, compared with the model group, the neurological function score in the TCM treatment group and the acupuncture and drug combination group decreased (P<0.05), and the behavioral function score increased (P<0.05). Compared with model group, the Shannon index of TCM treatment group and acupuncture and drug combination group increased (P<0.05). The abundance of Firmicutes increased (P<0.05), and the abundance of Bacteroidetes and Proteobacteria decreased (P<0.05) the abundance of Clostridia increased (P<0.05), and the abundance of Gammaproteobacteria decreased (P<0.05). The abundance of Escherichia-Shigella and Bacteroides decreased (P<0.05) the abundance of lactobacillus significantly increased (P<0.05). Conclusion Acupuncture combined with Buyang Huanwu Decoction can improve the symptoms of cerebral hypoperfusion model rats with carotid artery stenosis, and the mechanism may be to increase the abundance of probiotics.
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