Study on the correlation between the active components of Salviea Miltiorrhizae Radix et Rhizoma screened by high-throughput sequencing and the regulation of lncRNA-mRNA in human lung adenocarcinoma A549 cells
Objective Study on the correlation between the active components of Salviea Miltiorrhizae Radix et Rhizoma screened by high-throughput sequencing and the regulation of lncRNA-mRNA in human lung adenocarcinoma A549 cells.Methods A549 cells were cultured,and the IC50 dose of cryptotanshinone and tanshinone Ⅱ A was confirmed according to the cell proliferation experiment.A549 cells were randomly divided into blank control group,cryptotanshinone group,and tanshinone ⅡA group using a random number table method.After 24 hours of intervention,the cell cycle was detected by flow cytometry.High-throughput sequencing technique was used to detect the expressions of lncRNA and mRNA in A549 cells in intervention group and non-intervention group.By analyzing the expression profiles of differential genes related to cryptotanshinone and tanshinone Ⅱ A,the obtained differential genes were analyzed by GO and KEGG.Results The cell cycle results showed that the proportion of G0/G1 phase cells in cryptotanshinone and Tanshinone Ⅱ A was increased(P＜0.01),the proportion of S phase cells was decreased(P＜0.01),and the proportion of G2/M phase cells in cryptotanshinone was decreased(P＜0.01).The results of high-throughput screening showed that cryptotanshinone could up-regulate 4 698 lncRNA,down-regulate 1 557 lncRNA,up-regulate 4 810 mRNA and down-regulate 5 644 mRNA.Tanshinone Ⅱ A could up-regulate 1 348 lncRNA,down-regulate 1 299 lncRNA,up-regulate 4646 mRNA and down-regulate 4 741 mRNA.The function and pathway enrichment analysis of differential lncRNA and mRNA showed that the differentially expressed genes of cryptotanshinone and tanshinone Ⅱ A were mainly related to cell cycle,autophagy,AMPK signaling pathway,FoxO signaling pathway and EGFR signaling pathway.GAS5 may be one of the targets for the inhibitory effects of cryptotanshinone and tanshinone Ⅱ A.Conclusion Cryptotanshinone and tanshinone Ⅱ A have certain inhibitory effects on A549 cells,and there are differentially expressed genes of lncRNA-mRNA,which are closely related to cell cycle and signal pathway.
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