Analysis of effect of Epimedium on cells endogenous metabolites before and after the processing based on UPLC-Q-Exactive Orbitrap-MS
Objective To analyze the effects of processed Epimedii Folium on endogenous metabolites of mouse melanoma cells(B16 cells)before and after processing based on cell metabolomics;To investigate the changes of processed Epimedii Folium before and after processing.Methods Ultra performance liquid chromatography tandem four-stage orbital trap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)technology was used,and the endogenous small molecules of B16 cells treated with Epimedii Folium and processed Epimedii Folium were analyzed by metabolomics.The differential metabolites between groups were obtained,and relevant metabolic pathways were analyzed based on the MetaboAnalyst 5.0 database.Results Significant changes were observed in 13 kinds of endogenous metabolites,including alanine,carnitine C3∶0,glutamic acid-1,lactic acid,isoleucine,choline,phosphatidylcholine(34∶2,36∶2),free fatty acids,citric acid,carnitine C4∶0,lysophosphatidylcholine 16∶0 and malic acid after the intervention of Epimedii Folium and processed Epimedii Folium.And the impact of processed products on differential metabolites was stronger than that of raw products.The main pathways involved were Warburg effect,pyruvate metabolism,malate-aspartic acid shuttle,pyruvaldehyde degradation and so on.Conclusions Epimedii Folium and processed Epimedii Folium would have certain effects on cellular metabolic pathways.The results may be related to the pharmacological effects and changes in cold and hot properties of Epimedii Folium before and after processing.
Epimedii FoliumUltra high performance liquid chromatography tandem four bar orbital trap mass spectrometryEndogenous metabolites of cells
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