Study on the active components of nucleosides in Benincasae Exocarpium based on HPLC
Objective To establish HPLC characteristics of Benincasae Exocarpium from different regions;To simultaneously determine the contents of uridine,guanosine and other nucleosides;To conduct overall evaluation on Benincasae Exocarpium from different regions.Methods The chromatography was performed on Waters Atlantis®T3(250 mm×4.6 mm,5 μm)column with methanol(A)and aqueous solution(B)as mobile phase gradient elution.The detection wavelength was 254 nm,the flow rate was 1.0 ml/min,and the column temperature was 25 ℃.The similarity of common peak of content and characteristic map was used as the main evaluation index to analyze the difference of the Benincasae Exocarpium in different producing areas.Results 15 batches of Benincasae Exocarpium showed 6 common characteristic peaks,and the similarity with the control characteristic maps was greater than 0.90.5 components of guanine,xanthine,uridine,adenine and guanosine were identified,and uridine and guanosine were quantitatively determined by HPLC.The results showed that,the contents of uridine and guanyl in 15 batches of Benincasae Exocarpium ranged from 0.017％to 0.036％and 0.018％to 0.031％.The highest content of uridine was 0.036％in Jiaozuo City of Henan Province,and 0.031％in Changde City of Hunan Province.Conclusions This HPLC method has good precision,repeatability and stability,and the two components of uridine and guanosine have good linear relationship in the determination range,which can objectively and comprehensively evaluate the overall appearance of Benincasae Exocarpium from different regions.The difference of nucleoside efficacy components of Benincasae Exocarpium from different regions is small.
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