Qigui Fuyuan Decoction alleviating cerebral ischemia-reperfusion injury by attenuating neuroinflammation
Objective To explore the effects of Qigui Fuyuan Decoction(QGFYD)on improving neural function,reducing neuronal damage and inflammatory response in rats with cerebral ischemia-reperfusion injury.Methods A middle cerebral artery occlusion(MCAO)model was established in SD rats using suture occlusion method.The rats were divided into sham-operation group,model group,and QGFYD low-(QGFYD-L),medium-(QGFYD-M),and high-dosage(QGFYD-H)groups according to random number table method.The modified neurological severity scores(mNSS)was used to evaluate the neurological deficit score,Triphenyl tetrazolium chloride(TTC)staining to assess the volume of cerebral infarction,and Western blot for inflammatory factor expression and ELISA assay for serum inflammatory factor levels.Stimulation of PC12 cells with L-glutamic acid(L-Glu)was used to establish an in vitro neurological injury model,and QGFYD-containing serum and intestinal absorptive solution were prepared to study its neuroprotective effects in vitro using the CCK-8 assay kit.Results Compared with the model group,QGFYD-L,QGFYD-M,and QGFYD-H groups showed a decrease in neurological deficit symptom scores(P＜0.01),a decrease in cerebral infarction volume(P＜0.01),a decrease in serum IL-6 and TNF-α levels(P＜0.05),and a decrease in brain tissue IL-1 β,IL-6,iNOS,and TNF-α protein expression(P＜0.05);both the QGFYD-containing serum and the intestinal absorption solution could alleviate L-Glu induced damage to PC12 cells(P＜0.01).In vitro experiments showed that intervention with 30 mmol/L L-glutamate for 24 hours could cause damage to PC12 cells.Both the serum containing Qigui Fuyuan decoction and intestinal absorption solution could alleviate L-Glu-induced damage to PC12 cells(P＜0.01).Conclusion Qigui Fuyuan Decoction can alleviate cerebral ischemia-reperfusion injury,improve neurological function,and inhibit neuroinflammation.
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