Study on quality analysis of Lonicera alberti Regel.Leaves by QAMS
Objective To establish the QAMS method for content determination of eight chemical compositions(chlorogenic acid,neochlorogenic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C,rutin,morroniside and luteolin-7-O-glucoside)from Lonicera alberti Regel.leaves;To verify the feasibility and applicability of this method in quality control for Lonicera alberti Regel.leaves.Methods The HPLC analysis was performed on a Agilent Eclipse XDB-C18(250 mmx4.6 mm,5 μm)with a mobile phase consisted of acetonitrile and 0.1％formic acid solution in gradient elution manner at a flow rate of 1 ml/min.The column temperature was maintained at 30 ℃ and the detection wavelength was set at 258 nm.The injection volume was 10 μl.Results Chlorogenic acid,neochlorogenic acid,cryptochlorogenin acid,isochlorogenic acid A,isochlorogenic acid C,rutin,morroniside and luteolin-7-O-glucoside had a good linear relationship in the corresponding concentration range(r≥0.999 6).The average sample recovery rates were 103.16％,103.98％,99.49％,103.78％,102.74％,101.12％,104.62％,and 100.94％,respectively.The RSD values were 1.30％,1.63％,2.92％,2.10％,1.27％,2.40％,1.15％,and 2.76％,respectively.Chlorogenic acid was set as internal reference substance,the relative correction factors of isochlorogenic acid A,isochlorogenic acid C,neochlorogenic acid,cryptochlorogenin acid,morroniside,luteolin-7-O-glucoside,rutin were 0.785 5,0.693 9,1.001 5,1.087 2,1.233 9,0.369 1,0.507 5,respectively.The content determination results of QAMS method and external standard method showed that there was no statistical significance in the comparison of the other six components except for morroniside.Conclusions The established HPLC method can be used for the quality control of Lonicera alberti Regel.leaves.QAMS can be used to determine the contents of neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid C,rutin and luteolin-7-O-glucoside in Lonicera alberti Regel.leaves.
Honeysuckle LeafQuantitativeanalysis of multi-components with a single-markerRelative correction factorsAssay(TCD)Quality contral
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