Study on the mechanism of buyang huanwu decoction to improve diabetic peripheral neuropathy by regulating PI3K/Akt autophagy pathway
Objective To investigate the protective effect of buyang huanwu decoction on diabetic peripheral neuropathy(DPN)through phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)autophagy pathway.Methods Forty-eight Wistar rats were grouped into control group(gavage+intravenous sterile saline solution of equal volume),DPN model group(gavage+intravenous sterile saline solution of equal volume),traditional Chinese medicine treatment group(gavage 2 g/kg buyang huanwu decoction)and PI3K inhibitor group(gavage 2 g/kg buyang huanwu decoction+intravenous 1.5 mg/kg PI3K inhibitor LY2940002).Except for the control group,DPN rats in the other three groups were given the corresponding drug treatment.The conduction index and the ultrastructure of the sciatic nerve were observed.Serum factor expression levels and neuronal autophagy and apoptosis was analyzed.Expression levels of relevant proteins were examined.Results Compared with the control group,the sciatic nerve myelin axons were severely lost in DPN model group,the myelin sheath was destroyed and curled,the organelles were reduced,the speed and amplitude of the sciatic nerve conduction action sites were decreased,the levels of autophagy and apoptosis were increased,and the levels of PI3K/Akt pathway was inhibited,and the differences were statistically significant(P＜0.01).The above indexes were improved in the Chinese medicine treatment group,including ultrastructural changes,autophagy,apoptosis and PI3K/Akt pathway-related protein levels(P＜0.01).However,PI3K inhibitor partially reversed the protective effect of buyang huanwu decoction on DPN rat model(P＜0.01).Conclusion Buyang huanwu decoction may inhibit apoptosis and autophagy by activating the PI3K/Akt pathway,thereby exerting a protective effect on DPN rats.
buyang huanwu decoctionphosphatidylinositol 3-kinase/protein kinase B pathwayautophagydiabetic peripheral neuropathy
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