An investigation of the methodology for establishing a human-derived xenograft model of giant congenital melanocytic nevus
Objective To explore the establishment of a human-derived xenograft mouse model for giant congenital melanocytic nevus(GCMN).Methods Skin from GCMN patients was transplanted onto the backs of two mice with severe combined immunodeficiency,which were then removed after 4 weeks of pressure bandaging.The survival and histopathological characteristics of the transplanted GCMN skin were observed.Results Mouse No.l died at 1 week after operation,which may be related to overlarge graft area.Mouse No.2 survived for a long time.The skin xenograft was stable for at least 28 weeks.There was no scar formation at the junction of grafted skin and normal mouse skin.HE staining at GCMN site showed that there were epithelioid nevus cells and melanin granules in the dermis,and immunohistochemistry showed that melanin antigen,microphthalmia transcription factor,S-100 protein,Sry-related HMG-BOX gene 10 were all positive.Conclusion The human-derived xenograft mouse model of GCMN constructed by this method can maintain the histological features and important molecular phenotypes of GCMN.Meanwhile,the transplanted skin can survive for a long time,which can be used for subsequent basic experiments,and is conducive to the further investigation of the pathogenesis of GCMN and its potential drug development.
万方数据
维普
