Effects of flavonoids from moringa oleifera leaves on lipid metabolism in HepG2 cells and its mechanism
Objective To investigate the effects of flavonoids from moringa oleifera leaf on lipid metabolism in HepG2 cells and its potential mechanism.Methods HepG2 cell model with lipid deposition was constructed,and the cells were treated with moringa leaf flavonoids(final concentration 1,2,5,10pg/ml).Cell counting kit(CCK-8)assay was used to detect cell viability.Oil red O staining was used to detect intracellular lipid droplet staining.The expression of reactive oxygen species(ROS)and adenosine triphosphate(ATP)were detected by the kit.The expression of lipid metabolism-related genes[low-density lipoprotein receptor(LDLR),sterol regulatory component binding protein-1C(SREBP-1C),caveolin-1(CAV1)and acetyl coenzyme a acetyltransferase 1(ACAT1)]were detected by quantitative real time polymerase chain reaction.The expression levels of nuclearfactor-E2-relatedfactor(Nrf)2/hemeoxygenase(HO)-1 signaling pathway were detected by western blot.Results The cells treated with 1,2,5pg/ml of moringa leaf flavonoids were selected for the experiment.Compared with model group,the levels of ROS,SREBP-1c and AC AT1 in HepG2 cells in moringa leaf flavonoids treatment group were significantly decreased,while the levels of ATP,LDLR,CAV1 and Nrf2 and HO-1 protein expressions were significantly increased in a dose-dependent manner,with statistically significant differences(P＜0.05).Inhibition of Nrf2/HO-1 signaling pathway reversed the regulatory effect of moringa leaf flavonoids on lipid metabolism related genes expression.Conclusion Moringa oleifera leaf flavonoids can improve oxidative stress and lipid metabolism in lipid-deposited HepG2 cells,possibly through activation of Nrf2/HO-1 signaling pathway.
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