Study on the mechanism of kaempferol on adriamycin resistance sensitivity of lung cancer cells
Objective To explore the mechanism of kaempferol on adriamycin resistance sensitivity of lung cancer cells based on transforming growth factor β1/Ras related C3 botox substrate 1(TGF-β1/Rac1)signaling pathway.Methods H1299/R cells were divided into negative control group(NC),kaempferol low,medium and high-dose groups,TGF-β1 inhibitor group,Rac1 inhibitor group,kaempferol high-dose+TGF-β1 inhibitor group,kaempferol high-dose+Rac1 inhibitor group,and kaempferol high-dose+TGF-β1 inhibitor+Rac1 inhibitor group.MTT assay was used to detect the cell growth inhibition rate and drug resistance sensitivity.Flow cytometry was used to detect the cell apoptosis.Transwell chamber was used to detect the cell invasion.Western blotting was used to detect the expression of TGF-β1 and Rac1 protein.Results Compared with NC group,the half inhibitory concentration(IC50),cell invasion ability in kaempferol low,medium and high-dose groups,TGF-β1 inhibitor group,Rac1 inhibitor group were significantly decreased,while the cell apoptosis rate was increased,and the expression of TGF-β1 and Rac1 protein were significantly decreased(P＜0.05).Compared with the kaempferol high-dose group,the IC50,cell invasion ability,TGF-β1 and Rac1 protein expression of H1299/R cells in kaempferol high-dose+TGF-β1 inhibitor group,kaempferol high-dose+Rac1 inhibitor group,and kaempferol high-dose+TGF-β1 inhibitor+Rac1 inhibitor group were significantly decreased,while the cell apoptosis rate was significantly increased(P＜0.05).Conclusion Kaempferol can reverse adriamycin resistance in lung cancer cells,inhibit the invasion of adriamycin resistant cells and promote apoptosis,the mechanism may be related to the inhibition of TGF-β1/Rac1 signaling pathway.
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