首页|金宁方中β-谷甾醇增进槲皮素诱导肺癌A549细胞凋亡作用

金宁方中β-谷甾醇增进槲皮素诱导肺癌A549细胞凋亡作用

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目的 探讨β-谷甾醇联合槲皮素对A549细胞抑制作用以及机制.方法 将A549细胞分为对照组、槲皮素组、槲皮素+β-谷甾醇组、雌二醇组、雌二醇+槲皮素组以及雌二醇+槲皮素+β-谷甾醇组.采用细胞存活率检测A549细胞的抑制作用.采用流式细胞技术和免疫荧光法检测肺癌细胞的促凋亡情况.应用免疫印迹法检测磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶 B(protein kinase B,Akt)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路的调控作用.结果 槲皮素可抑制A549细胞生存率且具量效关系(P<0.05);雌二醇可促进A549细胞生长;与槲皮素组或槲皮素+β-谷甾醇比较,雌二醇+槲皮素+β-谷甾醇组更抑制A549细胞增殖,提高细胞凋亡比例及caspase-3的表达水平(P<0.05):促进mTOR和Akt磷酸化,减少P13K磷酸化.结论 在雌二醇干预下,β-谷甾醇联合槲皮素对A549细胞有抑制作用,其机制可能通过PI3K/Akt/mTOR通路对细胞凋亡起促进作用.
The effect of β-sitosterol in Jinning decoction on enhancing quercetin induced apoptosis of A549 cells of lung cancer
Objective To investigate the inhibitory effect of β-sitosterol on quercetin in A549 cells and its mechanism.Methods The A549 cells were divided into control group,quercetin group,quercetin+β-sitosterol group,estradiol group,estradiol+quercetin group,and estradiol+quercetin+β-sitosterol group.The cell survival rate the inhibitory effects of A549 cell.The flow cytometry and immunofluorescence methods were used to determine the pro-apoptotic effect of on lung cancer cells.Western blotting was used to the regulatory effects of phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway.Results Quercetin could inhibit the survival rate of A549 cells with dose-response relationship(P<0.05).Estradiol promoted the growth of A549 cells.Compared with quercetin group or quercetin+β-sitosterol group,estradiol+quercetin+β-sitosterol group more inhibited the proliferation of A549 cells,increased the proportion of apoptosis and the expression level of caspase-3(P<0.05),promoted the phosphorylation of mTOR and AKT,and decreased the phosphorylation of PI3K.Conclusions Under the intervention of estradiol,β-sitosterol combined with quercetin inhibited A549 cells,the mechanism of which may be through the PI3K/Akt/mTOR pathway of apoptosis.

β-sitosterolquercetinA549 cell of non-small cell lung cancer

俞杞泉、叶玉涵、吕春明、张泽良、杨扬、韩素静

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上海中医药大学附属龙华医院胸外科,上海 200032

上海中医药大学科技实验中心,上海 201203

上海中医药大学附属龙华医院老年医学科,上海 200032

β-谷甾醇 槲皮素 非小细胞肺癌A549细胞

上海市卫生健康委员会中医药科研项目

2020JP005

2024

世界临床药物
上海医药工业研究院

世界临床药物

CSTPCD
影响因子:0.849
ISSN:1672-9188
年,卷(期):2024.45(3)
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