Effects of ophiopogonin B on regulation of sonic hedgehog/glioma associated oncogene homolog 1 pathway on epithelial mesenchymal transformation and drug resistance in hepatocellular carcinoma
Objective To investigate the effects of ophiopogonin B on epithelial mesenchymal transformation(EMT),chemotherapy resistance and sonic hedgehog(Shh)/glioma associated oncogene homolog(Gli)1 pathway in hepatocellular carcinoma.Methods Screening suitable concentration of ophiopogonin B.The HepG2 cells were divided into control group,ophiopogonin B low(group A),medium(group B),high concentration group(group C)and ophiopogonin B high concentration+Shh agonist group(group D).The HepG2/adriamycin(ADM)cells were divided into HepG2/ADM group,ADM group,ophiopogonin B group and ADM+ophiopogonin B group.HepG2 cell proliferation,apoptosis,EMT,Shh/Gli1 pathways and related protein expression were detected.HepG2/ADM cells were treated to detect cell proliferation,apoptosis,Shh and Gli1 proteins.Results Compared with the control group,5-ethynyl-2'-deoxyuridine(EdU)positive rate,the expressions of Ki67,B cell lymphoma(Bcl)-2,neural cadherin,vimentin,Shh and Gli1 in groups A,B and C decreased successively,while the apoptosis rate,Bcl-2 associated X protein(Bax)and cadherin expression in epithelial cells increased successively(P＜0.05).Compared with group C,Edu positive rate,Ki67,Bcl-2,neural cadherin,vimentin,Shh and Gli1 expressions in group D were increased,while apoptosis rate,Bax and cadherin expression in epithelial cells were decreased(P＜0.05).Compared with HepG2/ADM group,EdU positive rate,Shh and Gli1 expressions in ADM and ophiopogonin B group decreased,and apoptosis rate increased(P＜0.05).Compared with ADM and ophiopogonin B groups,Edu positive rate,Shh and Glil expressions were decreased in ADM+ophiopogonin B group,and apoptosis rate was increased(P＜0.05).Conclusion Ophiopogonin B inhibits EMT of HepG2 cells by inhibiting Shh/Gli1 pathway and reducing chemotherapy resistance.
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