Study on motor neurotoxicity of aconitine and miR-124 as a potential toxicity marker
Objective To investigate the potential of circulating microRNA(miR)-124 as a marker of motor neurotoxicity of aconitine.Method Forty-two rats were randomly divided into control group,aconitine 0.025 mg/kg(low dose)group and aconitine 0.05 mg/kg(high dose)group,14 rats in each group,and were given intravenous injection for 14 days.Functional observation combination was used to detect the change of motor behavior.The pathology of brain tissue was observed.The apoptosis of brain cells was detected.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-124 in brain and blood.The levels of neuron-specific enolase(NSE)and glial fibrillary acidic protein(GFAP)in blood were detected by enzyme-linked immunosorbent assay.Results Compared with the control group,the motor behavior of D14 in low dose group and D1 and D14 in high dose group showed easier cage movement,reduced head or body movement,and weakened reflexes after administration(P＜0.05).Brain striatum necrosis and glial cell dysplasia were observed in high dose group.The apoptosis of cerebellar granule cell layer and cerebral cortex was increased in low dose and high dose groups,and the expression level of miR-124 in brain tissue was increased(P＜0.01).The increase of circulating miR-124 was the earliest in high dose group at 1 h after Dl administration(P＜0.01).At 8 h after D1 administration,the increase of circulating NSE and GFAP in high dose group was the earliest(P＜0.01).Conclusion Compared with circulating NSE and GFAP,circulating miR-124 increased earlier in high dose group,which has the potential to detect the motor neurotoxicity of aconitine.
aconitinetoxicity marker of motor neurotoxicityneuron-specific enolaseglial fibrillary acidic proteinmicroRNA-124
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