The mechanism of Zhonghe decoction in the treatment of abscess type lymph node tuberculosis
Objective To explore the mechanism of Zhonghe decoction in the intervention of abscess type lymph node tuberculosis.Methods Cell counting Kit 8 method was used to detect the cytotoxicity of THP-1 in different concentrations of neutralization soup solution.The results were further sequenced by second-generation high-throughput RNA transcriptome sequencing technology,and the differentially expressed genes were screened,and the Gene Ontology functional annotation and pathway analysis of Kyoto Encyclopedia of Genes and Genomes were performed.Results Zhonghe decoction can reduce the expression of inflammatory factors,inhibit the pathway related to bacterial infection,reduce the response of phosphatidylinositol 3-kinase/protein kinase B signaling pathway,and inhibit the signaling pathway related to pyroptosis and autophagy.Conclusion Zhonghe decoction may interfere with lymph node tuberculosis by inhibiting cell pyrodeath by inhibiting phosphatidylinositol 3-kinase/protein kinase B signaling pathway.
Zhonghe decoctionphosphoinositide 3-kinase/protein kinase Babscess type lymph node tuberculosispyroptosis
NETL
NSTL
万方数据
