The mechanism of Jianpi Qinghua formula in improving catecholamine resistance under obesity:a study based on multilevel molecular analysis
Objective To explore the mechanism of Jianpi Qinghua formula in improving catecholamine resistance in obesity by network pharmacology and molecular docking techniques.Methods The main active components and related targets of Jianpi Qinghua formula were screened by the database of traditional Chinese medicine system pharmacology and analysis platform.Catecholamine resistance targets associated with obesity were screened by the online human Mendelian genetics and GeneCards databases.The network topology diagram of"Jianpi Qinghua formula-active ingredient-target"and the protein-protein interaction network diagram were constructed by Cytoscape3.7.1 software to identify key targets.The intersection targets were imported into DAVID database for bioinformatics analysis.AutoDock Vina was used for molecular docking to screen and further verify the core chemical components of the formula.Results A total of 79 active ingredients and 661 targets were screened in the formula.A total of 1 337 catecholamine resistance targets and 262 intersection targets were identified in obesity.The key targets were signal transducer and activator of transcription 3,caspase 3,B-cell lymphoma-2 and serine-threonine kinase 1(AKT1).The results of the analysis of the Kyoto Encyclopedia of Genes and Genome showed that the improvement of catecholamine resistance by Jianpi Qinghua formula in obesity was related to the neuroactive ligand-receptor interaction,phosphatidylinositol-3-kinase/AKT1 signaling pathway and cyclic adenosine monophosphate signaling pathway.Conclusion The core components of Jianpi Qinghua formula of the potential mechanism may be 4',5-dihydroxyflavone,baicalein and jaranol in improving catecholamine resistance in obesity.
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