Mechanism of cyanidin-3-O-glucoside on high glucose and high fat-induced islet β cell damage based on HGF/c-Met pathway
Objective To investigate the effects of cyanidin-3-O-glucoside on the damage of pancreatic islet β cells induced by high glucose and high fat and its mechanism.Methods Islet β cells were treated with different concentrations(10,20,30,40,and 50 μmol/L)of cyanidin-3-O-glucoside,and cell viability was detected by CCK-8 method.The experiment was divided into control group,model group,cyanidin-3-O-glucoside 10 and 50 μmol/L group,the cell viability of each group was detected by CCK-8 method,the insulin secretion of each group was determined by ELISA method,the level of ROS was detected by DCFH-DA fluorescent probe,the SOD and MDA were detected by colorimetry,the expression levels of HGF/c-Met pathway related proteins were detected by Western blotting.In the pathway study,c-Met inhibitor SU11274 was used to intervene,and the experiment was divided into control group,model group,cyanidin-3-O-glucoside group,and cyanidin-3-O-glucoside + SU11274 group,the cell vitality of each group was detected by CCK-8 method,and the insulin secretion of cells in each group was determined by ELISA method.Results Compared with control group,different concentrations of cyanidin-3-O-glucoside significantly increased the activity of pancreatic β cells(P<0.05).Compared with model group,the cell viability of cyanidin-3-O-glucoside 10 and 50 μmol/L group were significantly increased,and the level of insulin secretion was significantly increased,ROS level and MDA content were significantly decreased,SOD activity was significantly increased,HGF and p-c-Met/c-Met protein levels were significantly up-regulated(P<0.05),and the effect of cyanidin-3-O-glucoside 50 μmol/L group was better than that of cyanidin-3-O-glucoside 10 μmol/L group(P<0.05).After the intervention with c-Met inhibitor SU11274,compared with cyanidin-3-O-glucoside group,the cell viability and insulin secretion levels in cyanidin-3-O-glucoside + SU11274 group were significantly decreased(P<0.05).Conclusion Cyanidin-3-O-glucoside can enhance the activity of pancreatic β cells induced by high glucose and high fat,increase insulin secretion and inhibit oxidative stress damage,which is related to the activation of HGF/c-Met pathway.
cyanidin-3-O-glucosidehigh glucose and high fatislet β cellsHGFc-Met
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万方数据
维普
