Protective effect of vitexin on ferroptosis mediated by the Sirt1/FoxO1 pathway on nasal mucosal damage with allergic rhinitis in rats
Objective To investigate the protective effect and regulatory mechanism of vitexin on nasal mucosal damage with allergic rhinitis in rats.Methods SD rats were randomly divided into control group,model group,vitexin(3,6,and 12 mg/kg)group,and vitexin(12 mg/kg)+ EX527(5 mg/kg)with 10 rats in each group.Except for the control group,all other groups of rats were induced with ovalbumin to establish a rat model of allergic rhinitis.After the successful establishment of the model,the rats in each group were ip administered with the corresponding dose of drugs,and the rats in the control group and the model group were ip administered with the same amount of normal saline once daily for 14 consecutive days.30 Minutes after the end of the last administration,record the behavioral scores of rats in each group and observe the pathological and morphological changes of nasal mucosa tissue.Detect the levels of SOD,GSH,MDA,ROS,and Fe2+ in nasal mucosa tissue,as well as the expression of ferroptosis related proteins SLC7A11,GPX4,ACSL4,Sirt1,FoxO1,p-FoxO1,and Ac-FoxO1 proteins.Results Compared with model group,the behavioral scores of rats in vitexin groups were significantly decreased,and the nasal mucosa injury was significantly improved(P<0.05).The activities of SOD and GSH in rat nasal mucosa homogenate were significantly increased,while the contents of MDA,ROS and Fe2+ were significantly decreased(P<0.05).The protein expressions of SLC7A11,GPX4,Sirt1,and FoxO1 in rat nasal mucosa were significantly increased,while the protein expressions of p-FoxO1,Ac-FoxO1,and ACSL4 were significantly decreased(P<0.05),and were dose-dependent.Conclusion Vitexin can improve nasal mucosal damage in allergic rhinitis rats,and its mechanism is related to activating the Sirt1/FoxO1 pathway and inhibiting ferroptosis in nasal mucosal tissue.
NETL
NSTL
万方数据
维普
