Mechanism of Tripterygium wilfordii in treatment of melanoma based on network pharmacology and experimentation
Objective To predict the active ingredients,targets and signaling pathways of Tripterygium wilfordii in treatment of melanoma by network pharmacology and experimentation,and to analyze the mechanism of Tripterygium wilfordii in treatment of melanoma.Methods To search the active components and targets of Tripterygium wilfordii from TCMSP database,and the intersection targets of traditional Chinese medicine and diseases were obtained from GeneCards and OMIM databases.Topological analysis was carried out on Cytoscape 3.7.1 to construct the"drug active ingredients-disease targets"network diagram.The interaction network and bar graph of common target protein were constructed with String platform,and the core target was screened out.Target gene enrichment analysis was carried out to complete the molecular docking of core targets and components.CCK8 and qPCR methods were used to verify the effects of core monomer components on melanoma and therapeutic targets.Results There were 51 active components of Tripterygium wilfordii.The best ingredient are kaempferol and triptolide.It has a significant inhibitory effect on melanoma cells,and significantly reduces the mRNA expression of core therapeutic targets Akt1 and VEGFA.Conclusion Tripterygium wilfordii has the characteristics of multi-target and multi-pathway in treatment of melanoma,and triptolide is its core medicinal component.This study provides experimental basis for Tripterygium wilfordii anti-melanoma.
NETL
NSTL
万方数据
维普
